Morphological, Molecular, and Pathological Appraisal of Hymenolepis nana (Hymenolepididae) Infecting Laboratory Mice (Mus musculus). Microsc Microanal

Journal Article
R, 2-Al-Olayan E1, Maha H. Elamin, Alshehri E1, Aloufi A1,2, Alanazi Z1, Almayouf M1, Bakr L3, Abdel-Gaber . 2020
Publication Work Type
Research
Publication Online URL
Tags
original, article
Magazine \ Newspaper
Microsc Microanal
Issue Number
20
Volume Number
16
Pages
1-16
Conference Date
Publication Abstract

Abstract
Hymenolepis nana, typically a parasite found in conventionally established mouse colonies, has zoonotic potential characterized by autoinfection
and direct life cycle. The objective of this study was to determine the rate of parasite infection in laboratory mice. The hymenolepidide
cestode infected 40% of the 50 mice sampled. The rate of infection in males (52%) was higher than in females (28%). Morphological
studies on the cestode parasite showed that worms had a globular scolex with four suckers, a retractable rostellum with 20–30 hooks, and a
short unsegmented neck. In addition, the remaining strobila consisted of immature, mature, and gravid proglottids, irregularly alternating
genital pores, lobulated ovaries, postovarian vitelline glands, and uteri with up to 200 eggs in their gravid proglottids. The parasite taxonomy
was confirmed by using molecular characterization based on the sequence analysis of the mitochondrial cytochrome c oxidase subunit 1
(mtCOX1) gene. The parasite recovered was up to 80% identical to other species in GenBank. High blast scores and low divergence
were noted between the isolated parasite and previously described H. nana (gb| AP017666.1). The phylogenetic analysis using the
COX1 sequence places this hymenolepidid species of the order Cyclophyllidea.