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أ.د. نايف بن سلطان الحربي

Professor

أستاذ البكتيريا الطبية / عضو هيئة تدريس في قسم النبات و الأحياء الدقيقة

كلية العلوم
كلية العلوم مبنى 5 مكتب 2ب 33
publication
Journal Article
2020

Solid state fermentation of amylase production from Bacillus subtilis D19 using agro-residues

, 24. Taghreed N. Almanaa, P. Vijayaraghavan, Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled and Sami A. Alyahya . 2020

Bacillus subtilis; Solid state fermentation; Amylase production; Agro-residues

The selected bacterial strain, Bacillus subtilis D19 was inoculated into the solid residues such as, wheat bran, banana peel, orange peel, rice bran and pine apple peel. Among the tested solid wastes, wheat bran showed enhanced the production of amylase (640 U/g) than other tested substrates. The carbon and nitrogen sources were initially screened by traditional method using wheat bran medium. Amylase activity was high in the wheat bran substrate supplemented with starch (670 U/g). The tested nitrogen sources enhanced amylase activity. Among the selected nitrogen sources, yeast extract stimulated maximum production of amylase (594 U/g). In two level full factorial experimental design, maximum activity (1239 U/g) was obtained at pH 9.0, 70% (v/w) moisture, 1% (w/w) starch, 1% (w/w) yeast extract and 5% (v/w) inoculums. Central composite design and response surface methodology was used to optimize the required medium concentration (pH, moisture content of the medium and starch concentrations) for the maximum production of amylase. All three selected variables enhanced amylase production over 3 fold in optimized medium. 

 

 

Publication Work Type
Article
Volume Number
2
Issue Number
32
Pages
1555-1561
more of publication
publications

The selected bacterial strain, Bacillus subtilis D19 was inoculated into the solid residues such as, wheat bran, banana peel, orange peel, rice bran and pine apple peel. Among the tested solid…

by 24. Taghreed N. Almanaa, P. Vijayaraghavan, Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled and Sami A. Alyahya
2020
publications

The body to build proteins can use L-Glutamic acid (L-GA). Earlier it was obtained from plant sources, later on microbes have been explored for the production. Corynebacterium glutamicum is a…

by 25. Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Taghreed N. Almanaa, Ganesh Moorthy Innasimuthu, Baskar Rajoo, Khalid F. Alanzi and Shyam Kumar Rajaram.
2020
publications

Background: Antibiotic-resistant bacteria are one of the major global health issues that can affect humans, animals, and the environment. Antibiotic-resistant bacteria have emerged as…

by Mohamed N. Alanber, Naiyf S. Alharbi and Jamal M. Khaled
2020