Optimization of glutamic acid production by Corynebacterium glutamicum using response surface methodology
, 25. Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Taghreed N. Almanaa, Ganesh Moorthy Innasimuthu, Baskar Rajoo, Khalid F. Alanzi and Shyam Kumar Rajaram. . 2020
The body to build proteins can use L-Glutamic acid (L-GA). Earlier it was obtained from plant sources, later on microbes have been explored for the production. Corynebacterium glutamicum is a prominent organism used to harvest the glutamic acid. Submerged fermentation was adopted for L-GA production. Various nitrogen/carbon substrates used to find out the best nitrogen and carbon source. Statistical methods especially RSM (response surface method) stayed employed for the effect of various factors such as inoculum size, urea, glucose and salt on the L-GA production. As per the preliminary studies, urea and glucose were chosen as carbon and nitrogen sources. Further, the optimum values have been maximally documented in the glucose (50 g/L), then urea (10 g/L), 19.24% of salt solution and 5% of inoculum size. Maximum Yield of L-GA is produced through RSM-16.49 g/L. The experimental L-Glutamic acid production was 16.36 g/L at an optimum condition that compared well to the maximum predicted values by RSM (16.499 g/L). Non-linear regression quadratic model was developed for the L-GA synthesis; the methodology was validated statistically and the determination coefficient (R-2) was found to be 0.991. Thus the study identified the potential carbon and nitrogen source for a higher yield for L-GA using C. glutamicum under submerged fermentation and also this method minimizes the time for optimizing the medium components statistically.
The selected bacterial strain, Bacillus subtilis D19 was inoculated into the solid residues such as, wheat bran, banana peel, orange peel, rice bran and pine apple peel. Among the tested solid…
The body to build proteins can use L-Glutamic acid (L-GA). Earlier it was obtained from plant sources, later on microbes have been explored for the production. Corynebacterium glutamicum is a…
Background: Antibiotic-resistant bacteria are one of the major global health issues that can affect humans, animals, and the environment. Antibiotic-resistant bacteria have emerged as…