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Dr. Javed Ahmad

Assistant Professor

Assistant Professor

كلية العلوم
Room # 2B 121, Second Floor, Bld # 5, Department of Zoology, College of Science
publication
Journal Article
2023

Artemisia monosperma induces ROS-mediated cell death in human colorectal carcinoma cells via modulating apoptotic genes

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in traditional medicine. Plant extracts are well documented to combat against cancer diseases. Nevertheless, the out-come of A. monosperma extracts on human colorectal carcinoma cells (HCT-116) have not been investi-gated in details.
Objectives: Thus, herein we aimed to evaluate the cytotoxic effect of different extracts i.e. n-hexane (AM-H), chloroform (AM-C), n-butanol (AM-B), and aqueous (AM-Aq) of A. monosperma against HCT-116 cells and to identify molecular mechanism(s) involved in cytotoxicity. Methods: The HCT-116 cells were trea-ted with 10–1000 lg/mL of extracts for 24 h.
Results: Our results displayed that all extracts significantly decreased cell viability in HCT-116 cells and changed the cellular morphology in a dose-dependent way. Among these extracts, AM-C showed higher cytotoxic activity with IC50 of 250.5 lg/mL. Further AM-C extract increased ROS production and reduced MMP level in HCT-116 cells. Moreover, AM-C extract induces cell apoptosis by the upregulation of proapoptotic related genes (p53, Bax, caspase-3, and caspase-9) and downregulation of antiapoptotic gene, Bcl-2. More significantly, these results revealed that AM-C extract enhances apoptosis in HCT-116 cells associated with ROS generation and mitochondrial dysfunction.Conclusion: Our findings suggest that the extracts of A. monosperma can be an auspicious therapeutic agent against colorectal cancer.

Publisher Name
ELZEVIER
more of publication
publications

Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…

by Maqsood A. Siddiqui , Rizwan Wahab , Quaiser Saquib , Javed Ahmad , Nida N. Farshori , Ebtesam S. Al-Sheddi , Mai M. Al-Oqail , Shaza M. Al-Massarani , Abdulaziz A. Al-Khedhairy.
Published in:
ELZEVIER
publications

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…

2023
Published in:
ELZEVIER
publications

Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…

by Javed Ahmad a, Rizwan Wahab, Maqsood A. Siddiqui, Quaiser Saquib, Abdulaziz A. Al-Khedhairy.
2023
Published in:
ELZEVIER