CLS 331 Clinical Biochemistry II
Course code: 331
Course name : Clinical Biochemistry II
Credit hours: 3 theory +1 practical =4
Level: 5
Course Objective:
To equip students with a broad understanding of the chemical and molecular events involved in biological processes. The Biochemistry provides a foundation for careers in medicine, biotechnology, or research in all branches of the biological sciences.
Courses Description:
Course work involves a study of the metabolic activity of animal tissue and its regulation. Major catabolic pathways related to carbohydrate, amino acid, and fatty acids are discussed in detail. The study includes a look at the bio-energetic of cells as well as the digestion and absorption of food material.
Course outline:
Laboratory Schedule:
Week | Subjects | Number of week | Number of hours |
1 | Laboratory Safety | 1 | 1 |
2 | The hydrolytic activity of salivary amylase on starch
| 1 | 1 |
3 | Quantitative determination of Amylase activity | 1 | 1 |
4 | The Production of Pyruvate and | 1 | 1 |
5 | ’Identification of free amino acids by TLC using silica gel plates’’ | 1 | 1 |
6 | Quantitative Determination of Protein by the Biuret &Lowery Reactions | 1 | 1 |
7 | Enzymatic digestion of fat by pancreatic lipase | 1 | 1 |
8 | Effect of Bile Salts on Pancreatic Lipase Activity | 1 | 1 |
9 | Determination of serum urea | 1 | 1 |
10 | The Colorimetric Estimation | 1 | 1 |
11 | Revision | 1 | 1 |
Methods of evaluation
Quizzes | 5% |
Reports | 5% |
Final practical Examination | 15% |
References:
1.David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, W. H. Freeman; 4th edition (April 23, 2004).
2. Robert K. Murray, Daryi K. Granner, Peter A. Mayes, Victor W. rodwell, Harpers Biochemistry; McGraw-Hill Publishing Co; 25th edition (August 28,1999)
3. J. G. Salway, Metabolism at a glance; Blackwell Publishers; 2nd edition ( August 15, 1999).