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صباح أحمد محمد الزهراني

Demonstrator

معيد

كلية العلوم
Address: Building 5, Floor 3, Office no. 5T251
publication
Journal Article
2022

Exploring the effect of silver nanoparticles on gene expression in colon cancer cell line HCT116

Exploring the effect of silver nanoparticles on gene expression in colon cancer cell line HCT116

Abstract: This study describes a new green method for silver nanoparticles (AgNPs) using Cymbopogon proximus (CP) extract and evaluates their potential anticancer properties
in HCT116 cells. Ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, and Fourier transforminfrared (FTIR) spectroscopy were used to
successfully analyze the AgNPs. FTIR spectral analysis revealed the presence of phytochemicals that could be responsible for silver (Ag) ion reduction and AgNP capping.
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay demonstrated that treating HCT116 cells with PCAgNPs for 48 h caused cytotoxic effects, as evidenced by the
existence of 20% cell viability. The RT-qPCR study revealed that the expression of two oncogenes (cathepsin B [CTSB] and epithelial cell adhesion molecule [EpCAM]) was significantly reduced in treated cells. The levels of various tumor
suppressor genes, including adenomatous polyposis coli (APC), Beclin1 (BECN1), nuclear translocation of β-catenin (CTNNB1), low-density lipoprotein receptor-related protein
6, LRP5, TP53, and TNF, were dramatically reduced in cells treated with CP extract, but this was not the case in cells treated with CP extract. To conclude, CP-AgNPs have demonstrated
their ability to induce cytotoxic action and exert antitumorigenic modulatory effects, particularly on the expression of CTSB and EpCAM in colon cancer cells,
utilizing AgNPs as an antitumor therapeutic agent for 48 h is not recommended, and reducing the treatment time could be more effective.

Publisher Name
Hussah M. Alobaid
Magazine \ Newspaper
Green Processing and Synthesis
Pages
9
more of publication
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Abstract: Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods,…

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Published in:
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Abstract

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2022
Published in:
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Abstract: This study describes a new green method for silver nanoparticles (AgNPs) using Cymbopogon proximus (CP) extract and evaluates their potential anticancer properties

by Hussah M. Alobaid, Maha H. Daghestani, Nawal M. AL-Malahi, Sabah A. Alzahrani, Lina M. Hassen, and Dina M. Metwally*
2022
Published in:
Hussah M. Alobaid