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أفراح فهد عبدالله الخريجي

Professor

أستاذ علم وظائف الاعضاء وبيولوجية التكوين

كلية العلوم
مبنى 5 الدور الثالث مكتب 112
publication
Journal Article
2021

Chlorogenic acid abates male reproductive dysfunction in arsenic-exposed mice via attenuation of testicular oxido-inflammatory stress and apoptotic responses

study, the protective potential of chlorogenic acid (CGA), a caffeoylquinic acid isomer abundantly found in many
plants, was investigated against sodium arsenite (NaAsO2)-induced testicular dysfunctions. Adult male Swiss
mice were either administered NaAsO2 alone at 5 mg kg 1 or co-treated with CGA at 100 mg kg1 or 200 mg
kg 1 body weight for 4 weeks. Results showed that NaAsO2-treated mice exhibited marked declines in testes
weight, sperm count, and viability accompanied by decreases in sexual hormonal levels. Moreover, NaAsO2
toxicity evoked exhaustion of antioxidant markers (SOD, CAT, GPx, GR, and GSH), down-regulation of Nrf2
(nuclear factor erythroid 2–related factor 2) gene expression level, and elevations in malondialdehyde. Further,
elevations in inflammatory cytokines (IL-1, TNF-α, and IL-6) together with the up-regulation of pro-apoptotic
biomarkers (Bax and caspase- 3) and down-regulation of anti-apoptotic Bcl-2 were observed in NaAsO2 intoxication.
Immunohistochemical analysis of testis sections of NaAsO2-treated mice showed high caspase-3 expression.
These findings were well supported with testicular histopathological examination. However, pretreatment
of mice with CGA resulted in noteworthy improvements in testicular damage induced by arsenic in a dosedependent
manner possibly mediated by the Nrf2 signaling pathway. Conclusively, CGA counteracted arsenicinduced
testicular injury through its antioxidant, anti-inflammatory, and anti-apoptotic properties. Therefore,
CGA could serve as a favorable intervention in the alleviation of arsenic-induced reproductive toxicity.

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