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Dr Ali Daoud



كلية العلوم
Department of Zoology College of Science King Saud University, Riyadh, Saudi Arabia

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

PhD, Abdullah A. AlKahtane, PhD1 , Saud Alarifi, PhD1 , Ahmed A. Al-Qahtani, PhD2,3, Daoud Ali, PhD1 , Suliman Y. Alomar, PhD1 , Mohammed S. Aleissia, PhD4 , and Saad Alkahtani, . 2018

Most of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cypermethrin pesticide on cultured human hepatocarcinoma (HepG2) cells. The HepG2 cells were exposed to cypermethrin (0, 5, 15, 40 ng/mL) for 24 and 48 hours. We observed that cypermethrin caused cell death of HepG2 cells using 3-(4, 5-dimethylthiozolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase tests. Furthermore, cypermethrin reduced HepG2 cells viability in a time and dose dependent basis, that was probably mediated through the induction of reactive oxygen species (ROS) and apoptosis. An increase in ROS generation with a concomitant increase in expression of the proapoptotic protein Bcl-2 and cytochrome c and decrease in the antiapoptosis protein Bax suggested that a mitochondria-mediated pathway was involved in cypermethrin-induced apoptosis. These findings provide insights into the underlying mechanisms involved in cytotoxicity of cypermethrin in HepG2 cells.

Volume Number
Magazine \ Newspaper
Dose-Response: An International Journal
more of publication

Metal nanomaterials such as bismuth oxide nanoparticles (Bi2O3NPs) have been extensively used in cosmetics, dental materials,pulp capping, and biomedical imaging. There is little knowledge about…

by Ali Alamer1 & Daoud Ali2 & Saud Alarifi2 & Abdullah Alkahtane2 & Mohammed AL-Zharani3 & Mohamed M. Abdel-Daim2,4 & Gadah Albasher2 & Rafa Almeer2 & Nouf K. Al-Sultan2 & Abdulaziz Almalik1,5 & Ali H Alhasan1,5 & Saa

Silver nanoparticles (AgNPs) possess properties that are important for industrial and medical applications. This study is aimed to investigate intra-peritoneal toxicity of AgNPs at 26, 52 or 78 mg…

by Saud Alarifi, Daoud Ali, Mohammed A Al Gurabi & Saad Alkahtani

The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA…

by Saud Alarifi, Daoud Ali, Saad Alkahtani, and Rafa S. Almeer