Seema zargar

Prof. Seema Zargar
                 
Postal Address:                                                         Phone:            8051692
E-mail:            szargar@ksu.edu.sa
Department of Biochemistry
College of science, King Saud university
Prince Turkey Road
Riyadh; Saudi Arabia-11495
PROFESSIONAL QUALIFICATIONS: Ph.D Biotechnology. 
RESEARCH TOPIC  of PhD: Molecular mechanism of pathogenesis of dengue virus and its interaction with host cell proteins.
SUMMARY OF THESIS
 Purification of recombinant envelope protein of Dengue virus  (infective domain) was done by Ni-NTA affinity column and it was labeled and interacted with blotted  two dimensional gels of total proteins of mammalian cells. This interaction could show us the proteins that might be responsible for DEN-virus entry or its propagation in mammalian cells. The principal techniques used in the study were Isolation of total proteins, one dimensional gel electrophoresis, two dimensional gel electrophoresis, far Western blotting, Ni-NTA protein purification of expressed proteins, electron microscopy and MALDI-TOF analysis, etc 
WORK EXPERIENCE:
Academic editor Biomed Research International 
2021- till date: Professor of Biochemistry at King Saud University Riyadh
2016-2020: Associate Professor at King Saud University. Riyadh.
 2011-2016: Assistant Professor at King Saud University. Riyadh.
 2009-2011: Associate Scientist at King Faisal Hospital and Research Centre, Riyadh.
 2004-2009: PhD Biotechnology, Jamia Hamdard. 
EXPERTISE DEVELOPED DURING Post doctorate
 FACS analysis of CD3, CD4 and CD8, CD25, CD44 and Ki67 antibodies.
 MACS separation of different cell types (Magnetic cell sorting)
 Adipose tissue separation and culturing from lipoaspirate of abdominal fat or gluteal fat tissue
 Elisa for IL-6, adiponectin, Insulin, leptin,TNF-a and other adipokines.
 Isolation of thymocytes and splenocytes from mice and their culture.
 Extraction of DNA from mouse tail and their genotypic.
 Extraction of protein and Western blotting of cell lysates.
 Culturing of different types of primary and secondary cultures including established cell lines.
 Gene expression by Reverse transcription PCR.
 Cloning, cDNA library preparation  and yeast two hybrid selection, Real time PCR, tunnel assay and double immunofluorescence.
 Supervising Trainees and students from other institutes like King Saud University, Alfaisal University, College of Pharmacy and Dentistry, etc.
 TEACHING EXPERIENCE
Topics Taught include: Biomembranes, Biochemistry of specialized tissues, Biotechnology and genetic engineering, Gene, Genetics, molecular carcinogenesis, Antibiotics, Immunology, Gene expression, bioinformatics, molecular Biology, selected Topics in Biochemistry, inborn errors of metabolism, Biochemistry of Blood.

 SUPERVISOR OF FOLLOWING THESES 
1. Analysis of the potential molecular effects of Tartrazine and high dose exposure of Aspirin on cell cycle in rat brain
2.Cross-omics analysis of neratinib induced effects on different tissues of mice
Effect of expression of STK11 and its promoter activity in human breast cancer cell lines. (PI)
Molecular Basis of Hereditary Spastic Paraplegia in Saudi Population (PI).
Role of Angplt8 Betatrophin gene polymorphism in Metabolic syndrome manifestations among Saudi subjects (PI).
The effect of Gum Arabic on cholesterol synthesis and serum lipids of atherogenic rats (PI).
Genetic screening of Saudi neonates for the neonatal diabetes associated mutations in ABCC8 gene (PI)

2.  Polymorphic pattern of certain genes in ovarian cancer in Saudi patients. (Co-PI)
3. Innate immunity and ovarian cancer: Role of the inflammatory cytokines (IL-1b  and TNF-a) and their receptors (IL-1 receptor and TNF receptor). (Co-PI)  
4. Anti-microbial activity of zingier officinal and curcum longa extracts against some selected micro-organisms’. (PI) 
5. Efficacy of medicinal plant extracts in growth of fungi and subsequent production of citrinin(PI) (selected in Scientific excellence for King Saud university students)
6. Assessment various impurities of water from several well water supplies from Riyadh region of Saudi Arabia (PI) .
7. Efficacy of date extract on inhibition of Citrinin production by Penicillium notatum and Aspergillus flavus (PI).
8. Cloning and sequencing of camel SOD gene (co-PI)  
Other Activities
Member, Postgraduate Committee                                                     2014-till date
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.   

Member, curriculum Committee                                                     2016-till date
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.   
 
Member, Accreditation Committee                                                     2016-2017
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.   
  
Member, Laboratory Committee                                                          2011-2013
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.   
                                                                                                    
Member, Examination Committee                                                        2011-2014
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.    

Member, Safety Committee                                                                  2011-2013
Department of Biochemistry, College of Science,        
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.   

 ACHIEVEMENTS: 
Scored second position in M. Sc Biochemistry 
Qualified JRF and Eligibility for Lectureship (NET) held on 22nd June 2003 conducted by Council of Scientific and Industrial Research, New Delhi (All India Level Exam).  
Senior Research Fellowship from Council of Scientific and Industrial Research (All India Level Eligibility Test).
Qualified GATE 2004 with 94.5 percentile at All India Level.
LIST OF PUBLICATIONS:

S Zargar, S Alamery, AH Bakheit, TA Wani. (2020) Poziotinib and bovine serum albumin binding characterization and influence of quercetin, rutin, naringenin and sinapic acid on their binding interaction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 235; 118335

TA Wani, AH Bakheit, Seema Zargar, H. Rizwana, A Al-Majed (2020). Evaluation of competitive binding interaction of neratinib and tamoxifen to serum albumin in multidrug therapy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. Q1.
TA Wani, AH Bakheit, Seema Zargar……..(2020) Study of binding mechanism of dapoxetine with Calf Thymus DNA by spectroscopic, thermodynamic techniques and molecular docking. South African journal of chemistry. Accepted.
Seema Zargar, Mona Alonazi, Humaira Rizwana, Tanveer A. Wani (2019) Resveratrol reverses thioacetamide-induced renal assault with respect to oxidative stress, renal function, DNA damage and cytokine release in Wistar rats. Oxidative Medicine and Cellular Longevity. Article ID 1702959. Q2
TA Wani, AH Bakheit, Seema Zargar, MA Bhat, AA Al-Majed. (2019) Molecular docking and experimental investigation of new indole derivative cyclooxygenase inhibitor to probe its binding mechanism with bovine serum albumin. Bioorganic chemistry 89, 103010. Q1
AA Al-Mehizia, AH Bakheit, Seema Zargar, MA Bhat, MM Asmari, TA Wani (2019) Evaluation of Biophysical Interaction between Newly Synthesized Pyrazoline Pyridazine Derivative and Bovine Serum Albumin by Spectroscopic and Molecular Docking Studies. Journal of Spectroscopy; Article ID 3848670. Q3
Nouf Alsalman, Abdulaziz Aljafari, Tanveer A. Wani, Seema Zargar (2019). High-dose aspirin reverses tartrazine-induced cell growth dysregulation independent of p53 signaling and antioxidant mechanisms in rat brain. Biomed Research International; Article ID 9096404. Q2
Tanveer A Wani, Ahmed H Bakheit, Mohammed Nazam Ansari, Abdul-Rahman A Al-Majed, Bakr M Al-Qahtani, Seema Zargar (2018). Spectroscopic and molecular modeling studies of binding interaction between bovine serum albumin and roflumilast. Drug Design Development and Therapy 12; 2627—2634.   Q2
Seema Zargar, AA TA Aljafari, Wani (2018).  Variants in MEF2A gene in relation with coronary artery disease in Saudi population. 3 Biotech. Jul;8(7):289. doi: 10.1007/s13205-018-1312-1. Q3
Seema Zargar, A A. Al-Majed and Tanveer A. Wani (2018). Potentiating and synergistic effect of grapefruit juice on the antioxidant and anti-inflammatory activity of aripiprazole against hydrogen peroxide induced oxidative stress in mice. BMC Complement Altern Med. 2018 Mar 23; 18(1). 106.  Q1
T A Wani, AH Bakheit, MA Abounassif, Seema.Zargar (2018). Study of Interactions of an Anticancer Drug Neratinib with Bovine Serum Albumin: Spectroscopic and Molecular Docking Approach. Front Chem. 2018 Mar 7; 6:47. Q2
Asma Alkaf, Abdulaziz Al-Jafari, T A. Wani, S Alqattan, Seema Zargar (2017). Expression of STK11 gene and its promoter activity in MCF control and cancer cells. 3 Biotech 7:362. DOI 10.1007/s13205-017-1000-6.
Tanveer A Wani*, Seema Zargar, Mohammed A. Hamidaddin,Ibrahim A. Darwish. Spectrophotometric and molecular modelling studies on in vitro interaction of tyrosine kinase inhibitor linifanib with bovine serum albumin. Plosone. Published:April18,2017.  https://doi.org/10.1371/journal.pone.0176015.
T A. Wani , AH. Bakheit , A Al-Majed, MA Bhat  and Seema Zargar. Study of the Interactions of Bovine Serum Albumin with the New Anti-Inflammatory Agent 4-(1,3-Dioxo-1,3-dihydro-2H-isoindol-2-yl)-N′-[(4-ethoxy-phenyl)methylidene]benzohydrazide Using a Multi-Spectroscopic Approach and Molecular Docking. Molecules 2017, 22(8),1258; doi:10.3390/molecules22081258. 
Seema Zargar, T A. Wani, A A Alamro, MA Ganaie. (2017). Amelioration of Thioacetamide Induced Liver Toxicity in Wistar Rats by Rutin.   
Seema Zargar , N J Siddiqi, S Ansar, M Alsulaimani & A. El ansary (2016). Therapeutic role of quercetin on oxidative damage induced by acrylamide in rat brain. Pharmaceutical Biology.
T A. Wani and Seema Zargar (2015). New ultra-performance liquid chromatographytandem mass spectrometry method for the determination of irbesartan in human plasma. Journal of food and drug analysis.  2 3; 5 6 9 -5 7 6.
Seema Zargar, T. A. Wani, S. K. Jain. (2015). Identification of Putative Vero Cell Protein(s) that Bind Specifically to Recombinant Envelope Protein of Dengue Virus Type 2. Tropical journal of Pharmaceutical Research. 14,(6) 997-1003.
T A. Wani , A.M. Alanazi, Seema Zargar, A. Ahmad, I. Darwish (2015). Box–Behnken Design Based Statistical Modelling for Optimization of UPLC-MS/MS Method for Analysis of Sorafenib in Bulk and Tablets. Journal of Computational and Theoretical Nanoscience. 12, 3598–3604.
T A. Wani and Seema Zargar (2015). New Highly-Sensitive Ultra-Performance Liquid Chromatography - Mass Spectrometry Method for Quantification of Telmisartan in Human Plasma. TJPR 14 (3): 511-518.
Seema Zargar, N JSiddiqi, S Al Daihan, TA. Wani. (2015) Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver. Acta biochimica polonica 62(2) 207-213.
Tanveer A. Wani, Seema Zargar and Ajaz Ahmad. Ultra Performance Liquid Chromatography Tandem Mass Spectrometric Method Development and Validation for Determination of Neratinib in Human Plasma. S. Afr. J. Chem., 2015, 68, 93–98
N JSiddiqi and  Seema Zargar (2015).. Quercetin protects mice kidney collagen against the damage induced by cadmium fluoride. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.
TA Wani and Seema Zargar. (2014).Sensitive UPLC-tandem mass spectrometric method for determination and validation of hydrochlorthiazide in human plasma. Lat. Am. J. Pharm, accepted on nov 21/ 2014.
Seema Zargar. (2014). Inhibitory effect of various aqueous medicinal plant extracts production and fungal biomass by Penicillium notatum and Aspergillus niger. IAIM. 1(3): 1-8.
Seema Zargar, NJ Siddiqi, TH Khan, IE Elredah. (2014). Effect of cadmium fluoride and quercetin on in vivo activity of indoleamine 2,3-dioxygenase in mice liver and kidney. Fluoride. 47(1): 31–42.
NJ Siddiqi and Seema Zargar (2014). Effect of quercetin on cadmium fluoride induced alterations in hydroxyproline/collagen content in mice liver. Connective tissue research. 55(3): 234-238.
Seema Zargar and TA Wani. (2014). Ultra-performance liquid chromatography/tandem mass spectrometry method for simultaneous determination of irbesartan and hydrochlorthiazide in human plasma. Accepted in Journal of the Iranian Chemical Society. DOI 10.1007/s13738-014-0429-3
Seema Zargar and T A. Wani. (2014). New UPLC-MS/MS method  for simultaneous determination of telmisartan and  hydrochlorthiazide in human plasma. Lat. Am. J. Pharm, 33(3);432-40.
Seema zargar. (2014). Protective effect of Trigonella foenum-graecum on thioacetamide induced hepatotoxicity in rats. Saudi Journal of Biological Sciences.
TA Wani and Seema Zargar (2015). Statistical modelling for optimization of automated spectrophotometric assay for determination of cefadroxil in its pharmaceutical formulations. Accepted in Lat. Am. J. Pharm
Tanveer A. Wani, Muzaffar Iqbal, Ibrahim Darwish Nasr Y. Khalil, Seema Zargar.(2014) Development and validation of sensitive uplc-ms/ms based method for the estimation of crizotinib in human plasma. Digest Journal of Nanomaterials and Biostructures. 9:693-704.
Perwez Alam, Mohammad S. Ahmad, Tanveer Ahmad Wani, Seema Zargar and Nasir A. Siddiqui.(2014).  Simultaneous quantification of two bioactive flavonoids Homoeriodictyol and Persicogenin in the methanol extract of aerial parts of two different species of genus Rhus by validated HPTLC densitometric method. Accepted in Journal of Planar Chromatography - Modern TLC October 2014. Manuscript No. JPC528R1
Seema zargar, S. wakil, A.  mobeirek, A. al‑jafari. (2013). Involvement of ATP‑binding cassette, subfamily A polymorphism with susceptibility to coronary artery disease. Biomedical Reports:, 883-888.
Darwish I A., Wani TA., Alanazi AM., Hamidaddin MA., Seema Zargar. (2013) Kinetic-exclusion analysis-based immunosensors versus enzyme-linked immunosorbent assays for measurement of cancer markers in biological specimens. Talanta. 111, 13-19.
S. Al-Daihan, M. Al-Faham, N. Al-shawi, R. Almayman, A. Brnawi, Seema zargar R. S. Bhat. (2013). Anti-microbial activity of zingier officinal and curcum longa extracts against some selected micro-organisms. Journal of King Saud University - Science, April, Volume 25, Issue 2, Pages 115-120.
T. A. Wani, A. Ahmad, Seema Zargar, Nasr Y. Khalil, Ibrahim A. (2012). Darwish. Use of response surface methodology for optimization and validation of new microwell-based spectrophotometric method for determination of atorvastatin calcium in tablets.  Chemistry Central Journal., 6:134 doi:10.1186/1752-153X-6-134.
Seema Zargar, Tanveer A. Wani, S. K. Jain. (2011). Morphological changes in vero cells postinfection with dengue virus type-2. Microscopy Research and Techniques.  74, 314-319.
T. A. Wani, M. Al-Omar, Seema Zargar. Huntington Disease: Current A dvances in Pathogenesis and Recent Therapeutic Strategies. International Journal of Pharmaceutical Sciences and Drug Research.  2011; 3(2): 69-79.
Seema zargar and S. K. Jain. (2008). Published several sequence in Uniprot KB/Swiss-Prot entry under accession no. p84856, p694580.
Seema and S.K.Jain, (2005). Molecular mechanism of pathogenesis of dengue virus: Entry and fusion with target cell. Ind. Jour. Clin. Biochem., 20 (2) 92-103. 
Book Chapters:
Colchicine induced ion channel formation into membranes as mechanism behind chemotherapy drug cytotoxicity of cancer cells. (2019) A. Alqarni, Seema Zargar, M. Ashrafuzamman. Chapter V, 151-189.Lipid bilayers. Nova medicine and health, New York.
Transcription and its regulation. (2017) Seema Zargar and Tanveer Ahmad Wani. Chapter II, 19-30. Quick Revision of Molecular Biology. Research India Publications. India. 

CONFERENCES AND WORKSHOPS:

1. Oral speaker  Genomic approach i treatment of viral diseases. in Genomics and Precision medicine. 
2. Poster in American chemical society.
3. Seema and S.K. Jain. Proteomic approach for characterization of cell surface receptors for dengue virus: protein profile of permissive and non-permissive cells. Biotechnology and Human welfare magazine. 2006. Hamdard University. New Delhi.
4. Seema and Jain S. K. proteome profile of permissive and non-permissive cells for different serotypes of dengue virus. 33rd immunological society  proceedings. 2004, 341-342.
5. Attended Interdisciplinary Science Conference on Recent Trends in Research in Biological Sciences.
6. Attended National Conference on Electron Microscopy and Allied Fields and XXIX Annual Meeting of EMSI.

  REVIEWER 
Reviewer to Journal of Infectious disease and immunity, Intervirology, Indian journal of Biological Sciences, Hindawi publishing systems,Science international
 MEMBERSHIPS: 
Member of European Federation of Biotechnology. 
Member of Indian Immunological society 

PERSONAL DETAILS:

 Attended a computer literacy programme conducted by the Department of Electronics and Communication Engineering of REC, Srinagar during May- July, 2000. Ample knowledge of computers with command on Windows, MS Office and internet world
Gender                                               :           Female
 Marital Status                                    :           Married
 Hobbies                                             :           Travelling, & Internet surfing.
 Nationality                                          :           Indian
 Permanent Address                         :           House No.29, New Housing Colony, Butta Kadal, Lal Bazar , Srinagar , Kashmir, India -190006
   
 
 
Seema Zargar