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صالح حسين سالمين - Saleh H. Salmen

Associate Professor

عضو هيئة تدريس

كلية العلوم
قسم النبات و الاحياء الدقيقة / معمل 20 B2 الدور الثاني كلية العلوم مبنى 5 | الهاتف: 0114675806
publication
Journal Article
2022

Expression and preliminary characterization of the potential vaccine candidate LipL32 of leptospirosis

Pothiaraj Govindan · Packiyadass Manjusha · Konda Mani Saravanan · Vijayakumar Natesan · Saleh H. Salmen · Saleh Alfarraj · Milton Wainwright · Harshavardhan Shakila

Leptospirosis is a globally re-emerging infectious disease mainly for mammals. The infection is caused by the spirochete Gram-negative bacterium Leptospira interrogans, which affects animals and humans worldwide. In our previous studies, recombinant protein production has been obtained from the bacterial expression system. In this study, we have investigated the over expression of LipL32 and hGMCSF genes into yeast expression system for obtaining a high yield of recombinant protein production. Here, we described the yeast expression studies with several applications such as protein folding, fast growth, and post-translational modification. The expression studies were carried out in a novel protein expression system, the methylotrophic yeast Pichia pastoris KM71 strain. The LipL32, Green fluorescent protein (EGFP), and human granulocyte– macrophage colony-stimulating factor (hGMCSF) genes were cloned into pPIC9 yeast expression vector. The recombinant clones of pPIC9-EGFP-LipL32 and pPIC9-EGFP-hGMCSF-LipL32 were transformed into Pichia pastoris KM71 strain by electroporation. Media optimization and other physiological characters were studied for the transformed recombinant protein. The protein was then purified using a Ni–NTA column; meanwhile, the recombinant DNA constructs contain His-tag at the C- terminal end. Finally, the intracellular EGFP expression of pPIC9-EGFP-LipL32, and pPIC9-EGFP-hGMCSF-LipL32 in Pichia pastoris KM71 strain was confirmed by fluorescence microscopic analysis. Protein–protein dockings were done to study LipL32-Adjuvant (hGMCSF, hIgGFC, and hC3d) interactions. Furthermore, this docking analysis was shown better interaction between LipL32, and hGMCSF, which is also used for the enhanced vaccine potential against leptospirosis.

Publication Work Type
بحث علمي
Magazine \ Newspaper
Applied Nanoscience
more of publication
publications

Here, we describe the novel biosynthesis of silver nanoparticles (AgNPs) using the extract of Indigofera oblongifolia (Hasr) leaves.

by SALMEN, S. H. – ALWHIBI, M. S. – ALHARBI, S. A.
publications

The sediment marine samples were obtained from several places along the coastline of the Tuticorin shoreline, Tamil Nadu, India were separated for the presence of bioactive compound producing…

by Suresh Dhanaraj , Somanathan Thirunavukkarasu , Henry Allen John , Sridhar Pandian , Saleh H. Salmen , Arunachalam Chinnathambi , Sulaiman Ali Alharbi
2020
publications

Leptospirosis is a globally re-emerging infectious disease mainly for mammals. The infection is caused by the spirochete Gram-negative bacterium Leptospira interrogans, which affects animals and…

by Pothiaraj Govindan · Packiyadass Manjusha · Konda Mani Saravanan · Vijayakumar Natesan · Saleh H. Salmen · Saleh Alfarraj · Milton Wainwright · Harshavardhan Shakila
2022