Food Biotechnology (Lab course)

Basics

Experiment 1 : Food biotechnology techniques (Basic lab procedures, equipment’s, safety and food sampling and storage)

Experiment 2 :  Bioinformatics (Food pathogens related -genes, marker and single nucleotide polymorphism (SNP) analysis using online tools  )

 

Nucleic Acids

Experiment 3 :  Genomic DNA isolation and purification from food samples

Experiment 4 :  Spectrophotometric Determination (DNA quantification and purity)

Experiment 5 : Primer designing & Polymerase Chain Reaction (PCR)

Experiment 6 : Agarose gel electrophoresis of DNA

Experiment 7 : RFLP & DNA Molecular Size Determination

Proteins

Experiment 8 :  Quantitative determination of Total proteins by Bradford method

Experiment 9 :  SDS- Polyacrylamide slab gel electrophoresis

Experiment 10 : Protein Molecular weight Determination

Experiment 11 : Enzyme linked immunosorbent assay (ELISA)

Experiment 12 : Food Isoenzymes detection and analysis by Native PAGE