Food Biotechnology (Lab course)

Basics
Experiment 1 : Food biotechnology techniques (Basic lab procedures, equipment’s, safety and food sampling and storage)
Experiment 2 :  Bioinformatics (Food pathogens related -genes, marker and single nucleotide polymorphism (SNP) analysis using online tools  )
 
Nucleic Acids
Experiment 3 :  Genomic DNA isolation and purification from food samples
Experiment 4 :  Spectrophotometric Determination (DNA quantification and purity)
Experiment 5 : Primer designing & Polymerase Chain Reaction (PCR)
Experiment 6 : Agarose gel electrophoresis of DNA
Experiment 7 : RFLP & DNA Molecular Size Determination
Proteins
Experiment 8 :  Quantitative determination of Total proteins by Bradford method
Experiment 9 :  SDS- Polyacrylamide slab gel electrophoresis
Experiment 10 : Protein Molecular weight Determination
Experiment 11 : Enzyme linked immunosorbent assay (ELISA)
Experiment 12 : Food Isoenzymes detection and analysis by Native PAGE