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نوال أحمد عبدالرحمن العرفج

Professor

أستاذ دكتور في الكيمياء التحليلية/قسم الكيمياء/كلية العلوم

Sciences
المدينة الجامعية للطالبات - كلية العلوم (مبنى 5) - الدور 3 - مكتب 336
publication
Journal Article
2021

Electrochemical Sensors for Berberine Hydrochloride Determination in Commercial Products and Bio-Fluids

The present study describes the development of three electrochemical sensors conducted by the
interaction of berberine hydrochloride (BRB) with phosphotungestic acid (plastic membrane), sodium
tetraphenyl borate (coated wire), and ammonium reineckate (coated graphite) sensors. Under optimum
experimental conditions the effect of membrane content, type of solvent mediator, soaking time,
hydrogen ion concentration of the test solutions and the estimation of the analyte in the presence of
foreign species were studied. The data confirmed that the developed sensors gave potential responses
of 54.10 ± 0.5, 57.00 ± 0.5 and 59.00 ± 0.5 mV decade-1
at ambient temperature for (berberine–
phosphotungstate, BRB-PT), (berberine–tetraphenylborate, BRB-TPB) and (berberine–reineckate,
BRB-AR) sensors, respectively, over drug concentration ranges of 1.0×10-7
- 1.0×10-3
, 1.0×10-8
-
1.0×10-2
and 1.0×10-7
- 1.0×10-2 mol L-1 with lower detection limits 5.0×10-8
, 5.0×10-9
and 5.0×10-8
mol L-1
for the above suggested sensors, respectively. The suggested sensors provide high selectivity,
precise and sensitivity for the determination of BRB within the average pH range 3-9. Validity of the
method was performed for the suggested electrochemical probe following ICH guidelines. The
suggested sensors were favorably exploited for the estimation of BRB in commercial products and biofluids.

Publication Work Type
Original research
Publisher Name
ESG
Volume Number
16
Magazine \ Newspaper
International Journal of ELECTROCHEMICAL SCIENCE
Pages
1-13
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