Silicon Nanoparticles on the Enhanced Micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult.

Journal Article

2024

Published in: https://link.springer.com/article/10.1007/s12633-023-02714-x

Silicon nanoparticles (SiNPs) in Murashige and Skoog (MS) medium improved micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult. (Apocynaceae), a valuable and threatened medicinal plant. Nodal explant-derived shoots amplified (130 shoots/inoculum/culture vessel) on MS medium + 1.0 mg L−1 SiNPs and 1.0 mg L−1 6-benzylaminopurine (BAP) + 0.5 mg L−1 kinetin (Kn) + 0.1 mg L−1 indole-3-acetic acid (IAA). These shoots exhibited superior growth over control. The SiNPs-derived shoots developed functional stomata, cuticle, multi-layered mesophyll, hypodermis, increased density of cells in the cortex, and vascular elements in the leaf and stem. In contrast, shoots generated on the control were with non-functional stomata, underdeveloped mesophylls, and few dermal, ground, and vascular tissues. The shoots were amplified on SiNPs-amended medium rooted maximum (99%) than control (90.3%) on 1/4th MS + 3.0 mg L−1IBA (Indole-3-butyric acid). The rooted plants were acclimatized with ease and successfully transferred to the field. It is suggested that the addition of 1.0 mg L−1 SiNPs in the cultivation medium induced micro-morpho-anatomical features improving the micropropagation of H. indicus.

• Publisher Name: Springer Nature
• Volume Number: 16
• Magazine \ Newspaper: Silicon
• Pages: 775–782