Skip to main content
User Image

Dr. MIKHLID HAMMAD ALMUTAIRI د. مخلد بن حامد المطيري

Associate Professor

Faculty member in Molecular Genetics

كلية العلوم
College of Sciences No. 5, ground floor, Office No. AB 67
publication
Journal Article
2015

β-Catenin accumulation and S33F mutation of CTNNB1 gene in colorectal cancer in Saudi Arabia

Dar, Suliman Y. Alomar, Lamjed Mansour, Abdulwahab Abuderman, Afrah Alkhuriji, Maha Arafah, Saleh Alwasel, Abdel Halim Harrath, Mikhlid Almutairi, Paul Trayhyrn, Javid Ahmad . 2015

Several risk factors associated with colorectal cancer (CRC) have been identified including β-catenin/CTNNB1 hotspot mutations. The levels of β-catenin within a cell are regulated via phosphorylation of the N terminus of β-catenin by GSK-3β. Thus far three serines (S33, 37, 45) and one threonine (T41) are considered to be the substrates for GSK-3β phosphorylation. In the present investigation an attempt was made to study the role of β-catenin mutations in exon-3 in 60 colorectal cancer patients from Kingdom of Saudi Arabia (KSA). The hot spot mutation region of β-catenin exon 3 was evaluated in matched tumor and normal tissues using PCR and direct sequencing. Sequencing of exon 3 of the CTNNB1 gene revealed an activating mutation (S33F) in one of the tumor samples as compared to the normal tissue from the same patient where there was no such mutation found. Immunohistochemical staining showed the accumulation of β-catenin protein both in cytoplasm and in the nuclei of cancer cells as compared to normal tissue.

Volume Number
67
Issue Number
2
Magazine \ Newspaper
Polish Journal of Pathology
Pages
156-162
more of publication
publications

The tumor suppressor gene TP53 and its downstream genes P21 and MDM2 play crucial roles in combating DNA damage at the G1/S cell cycle checkpoint. Polymorphisms in these genes can lead to the…

by Mikhlid H. Almutairi , Bader O. Almutairi , Turki M. Alrubie, Sultan N. Alharbi, Narasimha R. Parine, Abdulwahed F. Alrefaei, Ibrahim Aldeailej, Abdullah Alamri, Abdelhabib Semlali
2021
publications

Background: Tumor suppresser gene (TP53) and its downstream genes (P21 and MDM2) play crucial roles in the regulation of DNA damage checkpoint (G1/S) of the cell cycle. Genetic polymorphisms in…

2019