تجاوز إلى المحتوى الرئيسي
User Image

Dr. MIKHLID HAMMAD ALMUTAIRI د. مخلد بن حامد المطيري

Associate Professor

Faculty member in Molecular Genetics

كلية العلوم
College of Sciences No. 5, ground floor, Office No. AB 67
المنشورات
مقال فى مجلة
2015
تم النشر فى:

β-Catenin accumulation and S33F mutation of CTNNB1 gene in colorectal cancer in Saudi Arabia

Dar, Suliman Y. Alomar, Lamjed Mansour, Abdulwahab Abuderman, Afrah Alkhuriji, Maha Arafah, Saleh Alwasel, Abdel Halim Harrath, Mikhlid Almutairi, Paul Trayhyrn, Javid Ahmad . 2015

Several risk factors associated with colorectal cancer (CRC) have been identified including β-catenin/CTNNB1 hotspot mutations. The levels of β-catenin within a cell are regulated via phosphorylation of the N terminus of β-catenin by GSK-3β. Thus far three serines (S33, 37, 45) and one threonine (T41) are considered to be the substrates for GSK-3β phosphorylation. In the present investigation an attempt was made to study the role of β-catenin mutations in exon-3 in 60 colorectal cancer patients from Kingdom of Saudi Arabia (KSA). The hot spot mutation region of β-catenin exon 3 was evaluated in matched tumor and normal tissues using PCR and direct sequencing. Sequencing of exon 3 of the CTNNB1 gene revealed an activating mutation (S33F) in one of the tumor samples as compared to the normal tissue from the same patient where there was no such mutation found. Immunohistochemical staining showed the accumulation of β-catenin protein both in cytoplasm and in the nuclei of cancer cells as compared to normal tissue.

رقم المجلد
67
رقم الانشاء
2
مجلة/صحيفة
Polish Journal of Pathology
الصفحات
156-162
مزيد من المنشورات
publications

Diarrhea is a serious health concern in livestock, affecting productivity and animal welfare. However, it remains a significant threat. Various practical therapies are being explored, such as…

بواسطة 1. Houkang Ding, Ming Li, Ning Ma, Shahid Ali Rajput, Mikhlid H. Almutairi, Bader O. Almutairi, Zhaoqing Han, Aituan Ma, Dengshan Shiau
2025
publications

Selenium nanoparticles (Se-NPs), a nanoscale form of selenium, and sodium selenite (SS), an inorganic selenium compound, were incorporated into poultry feed to assess their effects on…

بواسطة 3. Aimen Khalid, Sania Satti, Anqash Ayyub, Faisal Nawab, Muhammad Tahir, Shabana Naz, Mikhlid H. Almutairi, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Demilade Israel Ibiwoye
2025
publications

Aqueous extracts of Leea crispa used for producing silver nanoparticles (AgNPs) with the supplementation of the external capping substance, were determined by UV-Visible spectroscopy. The…

بواسطة 4. Tejshwini Doddamani, Balamuralikrishnan Balasubramanian, Siddappa Kakkalameli, Joseph Kadanthottu Sebastian, Manikantan Pappuswamy, Bader O. Almutairi, Mikhlid H. Almutairi, and Azharuddin Daphedar
2025