497 حين
وصف المقرر:
تدريب تطبيقي في علم الحيوان
دراسة بعض الأجهزة الخاصة في التطبيقات العملية اللي يستخدمها الدارس لعلم الحيوان
King Saud University
497 Zoo
First exam –First Semester (1433 – 1434)H
اسم الطالبة : الرقم الجامعي :
الأستاذة المشرفة :
Put (T) or (F) : #
1- In Elisa before incubation should be cover the plate by special transparent paper.(T) 1
2- During Karyotyping, we use cold hypertonic solution for harvesting and worm fix to keep the cell shape for sliding.(F) 1
3- In Elisa, to know the activity of the conjugated enzyme, we use the substrate to give product and this product is called "measureable product".(T) 1
4- In Elisa, we use blocking buffer to remove unbound reagent.(F) 1
8- In Karyotyping, during banding we use the fix to elaborate dark and light bands of chromosomes.(F) 1
9-
(T) 1
Fill in the space: #
1-The steps of chromosome analysis method are…culturing..,..harvesting..,..sliding..,..banding..,and..staining. 2.5
2- In Elisa, insufficient washing gives…high….back ground, while excessive washing…decrease….the sensitively of antigen or antibody.1
3- In Karyotyping, the blood specimens are collecting in…hyparin…tape to keep the cell shape.1l2
4- During the first step of harvesting, we use…calcimade…to arrest the chromosome on …metaghase…1
5- The most widely used labeled enzymes in Elisa are…horse radish…or …AP…for their large selection of substrate.1
6- The most powerful Elisa is…sandwich…assay because it is robust is bound bet…two…primary antibodies,…caplure…and …detection…1.5
# Recount steps of:
1 1- How to extract DNA at home.
Peeling onions and then cut small pieces.
Add 200 ml of water and 2 teaspoons liquid and 2 tablespoons NACL and mix this mixture by a blender.
Put the mixture on the fire for a period of time.
filtrations for mixture.
Keep the filtrate with alcohol like Methanol 100% in a dish.
2 2- Giemsa stain:
Dissolve 5g of Giemsa power into 330ml of glycerin Mix.
Heat the solution from step 1 to 600C for 2 hour every 30min mix and shaking very well.
Leave solution at roome temperature to come cold then slowly add in 330ml of methanol to the solution from step 2 leave the solution one day.
Next day filter the solution from step 3.
The solution need to stand a period of time prior to use.
Leave for a week before use.
3- How to prepare Buffer in Karyotyping?2
Na2 HPO4 + KHPO4
500ml Distilled water-5,95 4,55-500ml Distilled water
Why we use Buffer in this method?
To keep the cell viability and to adjust ph for trypsin.
Chose the correct answer: #
1- Adentify :
A. chromosome number 2.
B. chromosome number 19.
C. X and Y chromosomes.1
2-The last step of chromosome elaboration method is:
A. Sliding.
B. Staining.
C. Harvesting.1
3-When the analyte is between competitive antibody and capture antibody, Elisa assay is called:
A. Sandwich Elisa.
B. Competitive Elisa.
C. In Direct Elisa. 1
4- In Elisa, the use of pipette:
A. Must touch the well.
B. Must not touch the well.
C. Stay contact with the well.1
5- . In Elisa, when we add the stop we should read the absorb:………………..
A. At once.
B. After time.
C. Leave the stop without reading.1