497 حين

وصف المقرر:

تدريب تطبيقي في علم الحيوان

دراسة بعض الأجهزة الخاصة في التطبيقات العملية اللي يستخدمها الدارس لعلم الحيوان

King Saud University

    497 Zoo

First exam –First Semester (1433 – 1434)H

اسم الطالبة :                                  الرقم الجامعي :

الأستاذة المشرفة :

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Put  (T) or (F) :                                                                                                         #                            

1- In Elisa before incubation should be cover the plate by special     transparent paper.(T) 1

2- During Karyotyping, we use cold hypertonic solution for harvesting and worm fix to keep the cell shape for sliding.(F) 1

3- In Elisa, to know the activity of the conjugated enzyme, we use the substrate to give product and this product is called "measureable product".(T) 1

4- In Elisa, we use blocking buffer to remove unbound reagent.(F) 1

8- In Karyotyping, during banding we use the fix to elaborate dark and light bands of chromosomes.(F) 1

9-

                                         (T) 1                                 

                                                                                                                              

 

 

 

 Fill in the space:                                                                                         #

 

      1-The steps of chromosome analysis method                     are…culturing..,..harvesting..,..sliding..,..banding..,and..staining. 2.5

2- In Elisa, insufficient washing gives…high….back ground, while excessive washing…decrease….the sensitively of antigen or antibody.1

3- In Karyotyping, the blood specimens are collecting in…hyparin…tape to keep the cell shape.1l2

4- During the first step of harvesting, we use…calcimade…to arrest the chromosome on …metaghase…1

5- The most widely used labeled enzymes in Elisa are…horse radish…or …AP…for their large selection of substrate.1

6- The most powerful Elisa is…sandwich…assay because it is robust is bound bet…two…primary antibodies,…caplure…and …detection…1.5

 

    # Recount steps of:

 

                                                              1 1- How to extract DNA at home.

Peeling onions and then cut small pieces.

Add 200 ml of water and 2 teaspoons liquid and 2 tablespoons NACL and mix this mixture by a blender.

Put the mixture on the fire for a period of time.

filtrations for mixture.

Keep  the filtrate with alcohol like Methanol 100% in a dish.

 

 

 

 

                                                                                              2 2- Giemsa stain:

Dissolve 5g of Giemsa power into 330ml of glycerin Mix.

Heat the solution from step 1 to 600C for 2 hour every 30min mix and shaking very well.

Leave solution at roome temperature to come cold then slowly add in 330ml of methanol to the solution from step 2 leave the solution one day.

Next day filter the solution from step 3.

The solution need to stand a period of time prior to use.

Leave for a week before use.

 

3- How to prepare Buffer in Karyotyping?2

Na2 HPO4                                   + KHPO4

    500ml Distilled water-5,95        4,55-500ml Distilled water

Why we use Buffer in this method?

To keep the cell viability and to adjust ph for trypsin.

 

 

 

 

 

 

 

 

 

 

                                                                                         

 

 

 

 

 

 

 

 

 

 

 

 

 

Chose the correct answer:     #

 

1- Adentify :

A. chromosome number 2.

B. chromosome number 19.

C. X and Y chromosomes.1

 

    2-The last step of chromosome elaboration  method is:

A. Sliding.

B. Staining.

C. Harvesting.1                         

 

    3-When the analyte is between competitive antibody and capture antibody, Elisa assay is called:

A. Sandwich Elisa.                                                                            

B. Competitive Elisa.                                                                        

 C. In Direct Elisa.      1

 

4- In Elisa, the use of pipette:

 

A. Must  touch the well.

B. Must not touch the well.

C. Stay contact with the well.1

 

5- . In Elisa, when we add the stop we should read the absorb:………………..

A. At once.

B. After time.

C. Leave the stop without  reading.1