How methyl cyanide induces aggregation in all-alpha proteins: A case study in four albumins
Khan, Priyankar Sen, Sadaf Fatima, Javed Masood Khan, Rizwan Hasan . 2009
Serum albumins are chief carrier of ligands in blood, hence important in clinical biotechnology. The effects of methyl cyanide (MeCN), a chief solvent of reverse phase chromatography, on four mammalian serum albumins (human, bovine, porcine and rabbit sources) were studied at neutral pH with the help of scattering, circular dichroism, IR and fluorescence spectroscopy. We have detected an intermediate state in the presence of 20% (v/v) MeCN, having 8–9% higher α-helical structure than that of their native states. In the presence of 60% (v/v) MeCN another intermediate was observed with non-native β-sheet structure and high tendency to form aggregates.
Serum albumins are chief carrier of ligands in blood, hence important in clinical biotechnology.
The interaction of bovine serum albumin (BSA) with cetyltrimethylammonium bromide (CTAB), C16C4C16Br2, Brij58, and their binary mixtures has been studied using tensiometry, spectrofluorometry, and…
This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana[Pa-2 (P.