Cross-Regulation between Autophagy and Apoptosis Induced by Vitamin E and Lactobacillus Plantarum through Beclin-1 Network

Journal Article

2022

Published in: https://www.mdpi.com/1986670

Abstract: Autophagy and apoptosis are two important regulatory mechanisms for how the body
can respond to diseases. This study was designed to investigate the protective actions of vitamin E
(Vit-E) and lactobacillus plantarum (Lac-B) against mercuric chloride (HgCl2)-induced kidney
injury. Thirty albino rats were divided into five groups: group 1 served as the normal group; rats in
group 2 received high doses of HgCl2; rats in groups 3, 4 and 5 were given Vit-E, Lac-B and the
combination of Vit-E and Lac-B, respectively along with HgCl2 for two weeks. HgCl2 provoked renal
injury, manifested by elevation in serum urea, urea nitrogen and creatinine. Kidney levels of
oxidative stress and inflammation were markedly increased post HgCl2 administration. Moreover,
HgCl2 significantly elevated the gene expression levels of VCAM-1 and cystatin C, while podocin
was downregulated. Additionally, it markedly decreased the protein expression of Beclin-1 and Bcl2. Histopathological examination revealed massive degeneration with congested blood vessels
following HgCl2 administration. Treatment with Vit-E or/and Lac-B restored the normal levels of
the previously mentioned parameters, as well as improved the morphology of kidney tissues. Both
Vit-E and Lac-B provided a protective effect against HgCl2-induced kidney damage by regulating
autophagy and apoptosis.

• Publication Work Type: بحث علمي
• Publisher Name: Int. J. Mol. Sci