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Muhammad Farooq khan

Associate Professor

Associate professor of Cell and molecular Biology

كلية العلوم
College of Science, building S5 Lab # 1B44 (zebrafish lab)

Epigenetic targets in hepatocellular carcinoma cells: identification of chaperone proteins complexes with histone deacetylases.

, 15. Muhammad Farooq and Mohammad A.M. Wadaan . 2013

HDA1 HDAC3 Liver cancer HepG2 affinity purification

The study was designed to find out the protein complex(s) associated with HDAC3 in liver cancer using a modified form of affinity purification coupled with a mass spectrometry technique in HepG2 cells. The organ-specific requirement for HDAC1 and HDAC3 during liver formation in zebrafish and their altered expression in liver cancer tissues indicates they are indispensible for hepato-organogenesis and hepatocarcinogenesis. However, how they exert their function is unknown.

MATERIAL & METHODS:

HepG2 cells were transfected with either mock or construct-containing HDAC3 using a C-terminal strepIII-HA tag as bait. The bait proteins were purified by double affinity columns and were analyzed on a Thermo LTQ Orbitrap™ (Thermo Scientific, MA, USA) chromatography system.

RESULTS:

Affinity purification coupled with mass spectrometry resulted in the identification of 24 putative binders of HDAC3 in HepG2 cells. The majority (83%) of these are novel interactions are reported for the first time in this study.

CONCLUSION:

This is the first study reporting the affinity purification and identification of protein complexes with two closely related proteins in one cell line. The novel HDAC1 and HDAC3 complexes identified in HepG2 cells could serve as a platform for the design of future therapeutic medicine for the treatment of liver cancer.

Publication Work Type
Research article
Volume Number
5
Issue Number
5
Magazine \ Newspaper
Epigenomics
Pages
501-12
more of publication
publications

The study was designed to find out the protein complex(s) associated with HDAC3 in liver cancer using a modified form of affinity purification coupled with a mass spectrometry technique in HepG2…

by 15. Muhammad Farooq and Mohammad A.M. Wadaan
2013
publications

Series of Schiff bases of valproic acid hydrazide,

N

-valproylglycine hydrazide and

N

-valproyl-4-ami

-

nobenzoyl hydrazide derivatives were synthesized…

by 13. Ayman El-Faham, Muhammad Farooq, Sherine N. KhattabAhmed M. Elkayal, Mahmoud F. Ibrahim, Nael Abu Taha, Mohammad A. M. Wadaan, Ezaat A. Hamed.
2014
publications

Pulicaria genus belongs to the family Compositae. Some species of this genus have been used in traditional Arab medicine as antispasmodic agent. In this study the Pulicaria glutinosa, which is a…

by 12. Nael Abutaha1, Muhammad Farooq, Fahd Abdu Al-Mekhlafi, Baabbad Almohannad, Sabir Shehata and Mohammad Wadaan
2014