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فهد عبده أحمد المخلافي

Associate Professor

قسم علم الحيوان

كلية العلوم
مبنى 5 الدور الأرضي رقم المكتب أب 75/1
publication
Journal Article
2022

Psiadia punctulata (DC.) Vatke induces cell apoptosis in highly metastatic MDA-MB-231 cells

Purpose: This study assessed the in vitro cell migration inhibitory and cell apoptotic effects of P.
punctulata stem (PPS (and leaf hexane) PPL (extracts on breast cancer cell lines (MDA-MB-231 and
MCF-7 cells).
Methods: Cytotoxicity was quantified using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) and lactate dehydrogenase (LD) release test after 48 h exposure of MDA-MB-231 cells to 0 –
200 μg/mL of PPS and PPL hexane extract. Cell apoptosis was determined using Muse™ cell
cytometry, while the phytoconstituents of PPS and PPL hexane extracts were identified by gas
chromatography–mass spectrometry.
Results: The half-maximal inhibitory concentration (IC50) for PPS and PPL hexane extracts against
MDA-MB-231 cells was 44.33 and 52.16 μg/mL, respectively. T, whereas the IC50 of PPS and PPL
hexane extracts was 102.22 and 59.53 μg/mL against MCF-7 cells, sequentially. Treatment with 100
and 200 μg/mL of PPS and PPL hexane extract increased late apoptosis in MDA-MB-231 cells to
16.005 ± 1.155 and 52.58 ± 3.02 %, respectively, for PPS hexane extract and 77.34 ± 0 % and 95.21 ±
1.61 %, respectively, for PPL hexane extract, when compared to control cells (3.81% ± 0.79%). PPL
hexane extract decreased cell migration and filled ~15.5 % of the wound gap on MDA-MB-231 cells
after 24 h, while PPS hexane extract decreased cell migration by ~35 and ~42.5 % at 24 and 48 h,
respectively. PPS and PPL hexane extracts contained several phytocompounds. Stem and leaf extracts
of P. punctulata showed significant (p < 0.05) cell apoptotic and migration inhibition activities.
Conclusion: The extracts P. punctulata exhibit potent cytotoxic activity against the tested breast cancer
cells. Further research is required to assess the acute and subacute toxicity of the extracts.

Volume Number
21
Magazine \ Newspaper
Tropical Journal of Pharmaceutical Research
Pages
769-777
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