Natural compounds have been used to treat cancer because of their low cytotoxicity and less adverse effects. Ginger has long been used in traditional medicine as it contains active phenolic compounds that have antioxidant, anti-cancer, anti-inflammatory, antiangiogenesis and antiartherosclerotic properties. We investigated the inhibitory activity of GO against colon cancer (Caco-2) cells viability or proliferation. Cells were incubated with GO at concentrations, 0.2%, 0.25, 0.3 to 0.35% of the volume of the media for 72 h at 37°C and 5% CO2. GO dose-dependently inhibited the viability and proliferation of colon cancer (Caco-2) cells in vitro. IC50 concentration of GO on treated cells inhibited proliferation and induced apoptosis even by DNA fragmentation besides caspase-3 reaction over expression. Future study will may deal with further investigations of the possible usages of GO as a new alternative chemotherapeutic agent specially for human colon cancer and other types of cancer