Antibacterial activity of selected medicinal plants of northwest Pakistan traditionally used against mastitis in livestock

Journal Article

2018

Published in: http://creativecommons.org/licenses/by-nc-nd/4.0/

, Rahila Amber, Muhammad Adnan, Akash Tariq, Shahid Niaz Khan, Sakina Mussarat, Abeer Hashem, Asma A. Al-huqail, Al-Bandari Fahad Al-Arjani, Elsayed Fathi Abd_Allah . 2018

a b s t r a c t
The present study aimed to investigate the efficacy of traditionally used anti-mastitis plants (Allium sativum,
Bunium persicum, Oryza sativa and Triticum aestivum) in northwest Pakistan against bacterial pathogens.
Selected plants were phytochemically screened for Alkaloids, Flavonoids, and Saponins and checked
for in vitro antibacterial activity at concentration of 50 mg/ml against S. aureus, E. coli and K. pneumoniae
by agar well diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration
was determined against multidrug resistant bacteria using tube dilution method. All extracts were
found to significantly inhibit (p < 0.01, p < 0.05) the activity against bacterial strains examined. Among
phytochemicals, alkaloids of all tested antimastitis plants produced significantly higher inhibition zones
against bacteria. The minimum inhibitory concentration and minimum bactericidal concentration of phytochemicals
and crude methanolic extracts against tested bacterial strains ranged between 12.5–50 mg/
ml and 25–50 mg/ml, respectively. Medicinal plants traditionally used against mastitis are therapeutically
active against bacterial pathogens. A. sativum and B. persicum were found to be potential candidate
species for the development of novel veterinary drugs with low cost and fewer side effects.

• Issue Number: 1
• Magazine \ Newspaper: Saudi Journal of Biological Sciences
• Pages: 154-161