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Javed Masood Khan

Assistant Professor

Researcher

كلية علوم الأغذية والزراعة
Department of Food Sciences, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
المنشورات
مقال فى مجلة
2010

Phytolacca americana lectin (Pa-2; pokeweed mitogen): an intrinsically unordered protein and its conversion into partial order at low pH

Khan, Ejaz Ahmad, Shah Kamranur Rahman, Javed Masood Khan, Ankita Varshney, Rizwan Hasan . 2010

This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana[Pa-2 (P. americana lectin-2)] can also be intrinsically unordered, based on the results obtained by CD, tryptophan fluorescence, ANS (8-anilinonaphthalene-1-sulfonic acid) binding, acrylamide quenching, DLS (dynamic light scattering) and its amino acid composition database analyses. Pa-2 is an acidic monomeric lectin and acquires random coil conformation at neutral pH without any regular secondary structure. As confirmed by different spectroscopic techniques, on lowering the pH, some secondary structures, predominantly α-helices, are detected by far-UV CD that adopt a marginally stable partially folded collapsed conformation possessing the characteristics of a premolten globule state. It is in accordance with coil–helix transition that is commonly observed when these intrinsically unordered proteins interact with their partner molecules in vivo.

رقم المجلد
30
رقم الانشاء
2
الصفحات
125-134
مزيد من المنشورات
publications

Serum albumins are chief carrier of ligands in blood, hence important in clinical biotechnology.

بواسطة Priyankar Sen, Sadaf Fatima, Javed Masood Khan, Rizwan Hasan Khan
2009
publications

The interaction of bovine serum albumin (BSA) with cetyltrimethylammonium bromide (CTAB), C16C4C16Br2, Brij58, and their binary mixtures has been studied using tensiometry, spectrofluorometry, and…

بواسطة Mohammad Amin Mir, Nuzhat Gull, Javeed Masood Khan, Rizwan Hasan Khan, Aijaz Ahmad Dar, Ghulam Mohammad Rather
2010
publications

This is the first report of its kind that well demonstrates that a lectin from Phytolacca americana[Pa-2 (P.

بواسطة Ejaz Ahmad, Shah Kamranur Rahman, Javed Masood Khan, Ankita Varshney, Rizwan Hasan Khan
2010