Dr.Subash Babu Pandurangan CV

Bio

I, Dr. P. SUBASH BABU, completed my Ph.D in Biochemistry. Presently working as an Assistant Professor in Biochemistry, King Saud University, Riyadh, KSA. Presently I am working on molecular diagnosis of metabolic disorders, specifically on obesity, diabetes and cardiovascular disorders. I have been investigating the role of microRNAs (miRNAs) in human Mesenchymal Stem Cells to adipocyte differentiation and adipogenesis, as well as other aspects of CVD and tumor development such as proliferation, survival and differentiation. MiRNA profiling particularly nutrient regulation of gene expression and expression of microRNA related to cell death (apoptosis and necrosis).
In addition I am investigating the food born carcinogens in cancer development in in Vitro and In Vivo models. Also I have been investigating the role of microRNAs (miRNAs) in cancer as well as other aspects of tumor development. Since, we have completed the current project on screening of miRNA for breast cancer, diabetes and obesity.

CV Text

Untitled document

Dr. P. SUBASH BABU Ph.D

Assistant Professor

Molecular Biology Research Lab -Nanobiotechnology unit

Department of Food science and Nutrition,

College of food science and agriculture,

King saud University

P.O. Box 2460, Riyadh 11451, KSA.

E-mail : subashbabu80@hotmail.com;

Mobile : +966-552563043

sbpandurangan@ksu.edu.sa Web : www.ksu.edu.sa

Objective A career, that is challenging and interesting. A job that gives me an opportunity to work at the leading edge of technologies, innovate and feel the satisfaction of success.

Areas of

Specialization Stem cell research, Molecular biology, Genetics, Diabetes, Clinical research, Cardio Vascular Biology, Drug development, Pharmacology and Biochemistry.

Education

Ph.D. BIOCHEMISTRY 2004 – 2009

Thesis title: Antidiabetic potential of Nymphaea stellata (Willd.) and Abelmoschus esculentus (Linn.).

M.Phil. BIOCHEMISTRY 2002 - 2004

Thesis title: Effect of hyponidd, an ayurvedic herbomineral formulation in normal and streptozotocin induced diabetic wistar rats.

M.Sc. BIOCHEMSITRY 2000 - 2002

B.Sc. BIOCHEMSITRY 1997 - 2000

Demographic Profile

Date of Birth: 31^st July, 1980

Gender: Male

Marital Status: Married

Nationality: Indian

Languages English (Read, write and speak)

Known: Tamil (Read, write and speak)

Hindi (Read and write)

Post Doctoral

Research Experience

2009 to till date, Working in Molecular Biology Research Lab, King Saud University, Riyadh, KSA.

Working as a project researcher in the area of “MicroRNA expression profiling of human mesenchymal stem cell adipogenesis and peptide mediated silencing by MPG nanoparticle - In Vitro” at King saud University, Riyadh, KSA.

Working as a Project fellow (KACST) in “ In-vitro screening of canrcinogenic nano food particles”- from-Aug’ 2010- till date.

Teaching

Experience Working at King Saud University, Riyadh, KSA.

Subject handled in King Saud University, Riyadh.

Clinical Nutrition, Basic Science – PG Students

Inborn Errors of metabolism - PG Women Students

Worked as a lecturer in Department of Biotechnology, Loyola College, University of Madras, Chennai from November 2007 to Sep 2009.

Subject handled in King Saud University, Riyadh.

Nutrition and metabolism, Enzymology, Immunology, Basic Sciences and Clinical biochemistry- for PG Students

Industrial

Experience Worked as a project manager in India diabetes research foundation, in a project entitled “Screening of IGT and Diabetics in south Indian population” from Feb’ 2009 to Dec’ 2009

Research Experience

2001 – 2009: Seven years of research experience as Ph. D (University of Madras), M.Phil (Annamalai University) Research Scholar and M. Sc Dissertation work (Bharadhithasan University) at Tamil Nadu, India.

Awards &

Honors Senior Research Fellowship (February 2006 to January 2008), awarded by Indian Council of Medical Research, New Delhi, India.

Patent

S. Ignacimuthu and P. Subash-Babu (2007). A process for preparation of a novel compound, Nymphayol [17-(hexan-2-yl)-10,13-dimehylhexadechydro-1H-cyclopenta[a]phenantheren-3-ol] from Nymphaea stellata willd. Flower with antidiabetic property. The patent office journal, 1567/CHE/2007 A.

Publications

M. Ayyanar, Pandurangan Subash-Babu, S. Ignacimuthu. (2013). Syzygium cumini (L.) Skeels., A Novel Therapeutic Agent for Diabetes: Folk medicinal and pharmacological evidences. Complementary Therapies in Medicine, CTIM-D-11-00025 (In press).

Ali A Alshatwi, Vaiyapuri Subbarayan Periasamy, Pandurangan Subash-Babu, Mohammed A. Alsaif, Abdulrahman A Alwarthan, Lei KA (2013). CYP1A and POR gene mediated mitochondrial membrane damage induced by carbon nanoparticle in human mesenchymal stem cells. Environmental toxicology and pharmacology (In press).

Amal Al-Hazzani, Vaiyapuri Periyasamy, Pandurangan Subash-Babu, Ali A. Alshatwi. (2012). Formulation of cashew nut shell liquid (CSNL) nanoemulsion, a potent inhibitor of human MCF-7 breast cancer cell proliferation. Med Chem Res. Volume 21, Issue 7, pp 1384-1388.

P. Subash-Babu and Ali A Alshatwi. (2012) Aloe-emodin inhibits adipocyte differentiation and maturation during in vitro human mesenchymal stem cell adipogenesis. Journal Biochemical and molecular Toxicology. J Biochem Mol Toxicol. 2012 Aug;26(8):291-300. doi: 10.1002/jbt.21415.

Periasamy, V.S., Subash-Babu, P., Muthukumaran, V.R. Akbarsha, M.A. Alshatwi, A.A. 2012. In Vitro Cytotoxic Effect of Formulated Semecarpus Ghee Nanoemulsion on Human Cervical Cancer (SiHa) Cells Advanced Science letters. Volume 6 2012 , pp. 75-79(5)

Alshatwi AA, Ramesh E, Periasamy VS, Subash-Babu P. The apoptotic effect of hesperetin on human cervical cancer cells is mediated through cell cycle arrest, death receptor, and mitochondrial pathways. Fundam Clin Pharmacol. 2012 Jun 29. doi: 10.1111/j.1472-8206.2012.01061.x.

P. Subash-Babu, Ali A Alshatwi,S. Ignacimuthu. (2012). Protection of pancreatic β-islets by cinnamaldehyde isolated from Cinnamonum zeylanicum Blume. Fundamental and clinical pharmacology (Accepted)

P. Subash-Babu, P. Antoni samy, Ali A Alshatwi, S. Ignacimuthu (2012) Anti-nociceptive, immunomodulatory and antipyretic activity of nymphayol isolated from Nymphaea stellata (Willd.) flowers. Biomolecules & Therapeutics. (Accepted).

Muniappan Ayyanar, Pandurangan Subash-Babu. 2012. Syzygium cumini (L.) Skeels: A review of its phytochemical constituents and traditional uses. Asian Pacific Journal of Tropical Biomedicine, 240-246.

P. Subash-Babu, Savarimuthu Ignacimuthu, Paul Agastian, Babu Varghese, (2009). Partial regeneration of beta-cells in the islets of Langerhans by Nymphayol a sterol isolated from Nymphaea stellata (Willd.) flowers. Bioorganic & Medicinal Chemistry 17 2864–2870.

P. Subash Babu, P. Prabuseenivasan, S. Ignacimuthu., (2007). Cinnamaldehyde- A potential antidiabetic agent. Phytomedicine. 14, 15–22.

P. Subash-Babu, S. Ignacimuthu, P. Agastian., (2008). Insulin Secretagogue effect of Ichnocarpus frutescence leaf extract in experimental diabetes: A dose-dependent study. Chemico- Biological interactions. 152, 171-179

P. Subash-Babu and S. Ignacimuthu, (2008). Antihyperlipidemic and Antioxidant effect of Hyponidd in the brain of streptozotocin induced diabetic rat. International Journal of Biological Chemistry. 1(4), 196-204.

P.Subash-Babu, S. Ignacimuthu and P.S. M. Prince, (2007). Restoration of altered carbohydrate and lipid metabolism by Hyponidd, a herbomineral formulation in streptozotocin induced diabetic rats. Asian Journal of Biochemistry. 3(2), 90-98.

P. Subash Babu and P. Stanely Mainzen Prince. (2004). Antihyperglycemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin induced diabetic rats. Journal of Pharmacy and Pharmacology. 56(11):1435-1442.

Molecular Biology

Techniques

Microarray-Nucleic acid and Protein array

FACS

DNA, RNA extraction and purification (chromosomal and extra chromosomal)

DNA and RNA quantification-Nanodrop

PCR based gene amplification

RT-PCR

Western blotting analysis

Hands-on Experience in Techniques

Restriction digestion analysis

Ligation and transformation

Selection of the clones using antibiotic markers

Conformation of the clones using PCR and REA

IPTG induction for the expression and purification

Isolation of cytosolic and nuclear fractions from tissue samples/cells.

RIA for Peptide and Steroid hormone assay

Hormone receptor study

Herbal drug standardization

HPLC

GCMS

ELISA

SDS-PAGE

Human Mesenchymal Stem Cell: proliferation and differentiation.

Cell culture maintenance for in vitro studies (Skin fibroblast, Detroit 573, Vero cells, Chang liver cells, Hep G2, COLO 320M, Adipocytes and Myocytes)

Antiobesity study- In vitro antiadipogenic effects using 3T3 L1 preadipocyte cell line

Cytotoxicity assay (MTT Assay)

Microbial culture

Trypan Blue assay

Cell counting

In Conferences

Saudi medicare, medical forum April 2010, 13^th International forum for hospital equipments and medical devices.

1^st International conference on biomarker research, organized by king saud University, January 2011.

4^TH International conference on biomarker research for cardiovascular research and diabetes, All Kozama hotel, organized by King saud university. (April 2010)

The first Indian congress on alternative to the use of animals in Research, Testing & Education, International conference Organize by Sri Ramachandra University and I-CARE, January, 2007.

Young Scientists’ conference on Environment, Health & Food. Organized by PG Research Department of Advanced Zoology and Biotechnology, Loyola College, Chennai. December, 2007.

Permanent address: Dr. P.Subash Babu

B.Manaveli, Boothavarayanpet & Post,

Bhuvanagiri via

Chidambaran Taluk

Cuddalore District

Tamilnadu, India. (Pin : 608 601)