Practical metabolism BCH 447

• Course: BCH 447 Practical Metabolism

ISOLATION OF GLYCOGEN FROM LIVER, ENZYMATIC and acid HYDROLYSIS OF isolated rabbit GLYCOGEN AND DETERMINATION OFGLUCOSE; Hydrolysis of protein: 1. time course for the hydrolysis of casein by proteolytic enzymes 2. time course for the hydrolysis of casein by concentrated HCL; Hydrolysis of fat or oils: 1. Determination of saponification number of fat or oil, 2. Lipase hydrolysis of at or oil; Localization and study of the properties of some dehydrogenases in mammalian tissues; Glycolysis in cell-free extract from yeast extract; Electron transport and oxidative phosphorylation by NAD-dependent oxidation of B-3hydroxybutyrate.