Skip to main content
User Image

منصور خليل محمد غطاشه

Assistant Professor

عضو هيئة تدريس

كلية العلوم
كلية العلوم، مبنى 5، قسم الكيمياء الحيوية، مكتب رقم (2أ 64)
Journal Article

Generation of Oxygen Free Radicals by Proflavine: Implication in Protein Degradation

Proflavine, an acridine dye, is a known DNA intercalating agent. In the present study, we show that proflavine alone on photoillumination can generate reactive oxygen species (ROS). These proflavinederived ROS cause damage to proteins, and this effect is enhanced when the divalent metal ion Cu (II) is included in the reaction. Bathocuproine, a specific Cu (I) sequestering agent, when present in the reaction mixture containing Cu (II), was found to inhibit the protein degradation, showing that Cu (I) is an essential intermediate in the reaction. The effect of several scavengers of ROS such as superoxide dismutase, sodium azide, potassium iodide, and thiourea were examined on the protein damaging reaction. Potassium iodide was found to be the most effective in inhibiting protein damage followed by sodium azide and thiourea. Our results indicate the involvement of superoxide, singlet oxygen, triplet oxygen, and hydroxyl radicals in proflavine-induced damage to proteins.

Publication Work Type
Research Article
Publisher Name
HAYATI Journal of Biosciences
more of publication

There is an immediate demand for eco-friendly, high-performance, and highly stable energy storage materials for pulse power systems. Ceramics based on SrTiO3 have a high breakdown strength (BDS)…

by Mahmoud S. Alkathy, Srinivas Pattipaka, Mansour K. Gatasheh, H. A. Kassim, Mohamed Saad Daoud and Jose A. Eiras
Published in:

Ceramics have enormous potential in several emerging technologies, including nuclear reactors. Materials with chemical inertness, high-temperature operation, and physical properties stability…

by Mansour K. Gatasheh, Mahmoud S. Alkathy, Hamoud Abdoh Kassim, Jakkapally Pundareekam Goud, Jose A. Eiras
Published in:
Open Chemistry

Proflavine finds a wide array of applications in clinical, therapeutic, industrial and cutting edge research. This study investigates properties of proflavine and brings out its current status…

by Mansour K. Gatasheh, S. Kannan, K. Hemalatha, N. Imrana
Published in:
Karbala International Journal of Modern Science