Skip to main content
User Image

منصور خليل محمد غطاشه

Assistant Professor

عضو هيئة تدريس

كلية العلوم
كلية العلوم، مبنى 5، قسم الكيمياء الحيوية، مكتب رقم (2أ 64)
Journal Article

Photo-activated proflavine degrades protein and impairs enzyme activity: Involvement of hydroxyl radicals

Proflavine is a well-known antiseptic and bacteriostatic drug, however, it has the potential to be hazardous and mutagenic. Proflavine enters cells and intercalates between DNA base pairs, resulting in mutation and replication inhibition. Previously several investigators demonstrated that photo-activated proflavine generated doublestranded
DNA breakage and protein structural alterations. The present study investigated the role of hydroxyl radical (⋅OH) due to activation of proflavine in the breakdown of protein and enzyme by photo-activated proflavine. The results show that the formation of hydroxyl radicals increased as the photo-illumination period increased, as did the concentrations of proflavine and Cu (II). As demonstrated by SDS-PAGE, the excess of free radicals due to proflavine resulted in oxidative modifications and degradation of BSA protein and trypsin enzyme. Additionally, with an increase in Cu (II) concentration, photo-illuminated proflavine induced a considerable loss of enzyme activity and also accelerated the degradation of the enzyme. Bathocuproine, a particular Cu (I)-sequestering agent, prevented protein degradation and enzyme inactivation. Hydroxyl radical
scavengers inhibited the protein-damaging process, indicating that hydroxyl radicals play a substantial role in protein damage. The tryptophan moiety was quenched by proflavine, demonstrating that it binds to proteins and enzymes, changing their structure and activity. As a result, this study helps to better understand proflavine’s deleterious influence on protein and enzyme degradation by oxygen-free radicals.

Publication Work Type
Research Article
Publisher Name
Toxicology Reports
more of publication

There is an immediate demand for eco-friendly, high-performance, and highly stable energy storage materials for pulse power systems. Ceramics based on SrTiO3 have a high breakdown strength (BDS)…

by Mahmoud S. Alkathy, Srinivas Pattipaka, Mansour K. Gatasheh, H. A. Kassim, Mohamed Saad Daoud and Jose A. Eiras
Published in:

Ceramics have enormous potential in several emerging technologies, including nuclear reactors. Materials with chemical inertness, high-temperature operation, and physical properties stability…

by Mansour K. Gatasheh, Mahmoud S. Alkathy, Hamoud Abdoh Kassim, Jakkapally Pundareekam Goud, Jose A. Eiras
Published in:
Open Chemistry

Proflavine finds a wide array of applications in clinical, therapeutic, industrial and cutting edge research. This study investigates properties of proflavine and brings out its current status…

by Mansour K. Gatasheh, S. Kannan, K. Hemalatha, N. Imrana
Published in:
Karbala International Journal of Modern Science