CLS 331

Department              :          Medical Sciences

Course Number      :           CLS 331

Course Title             :           Clinical Biochemistry ( II )   

Credit Hours            :           3 + 1 = 4

 

 

Course Description:

Course work involves a study of the metabolic activity of animal tissue and its regulation. Major catabolic pathways related to carbohydrate, amino acids, and fatty acids are discussed in detail . the study includes a look at the bioenergetics of cells as well as the digestion and absorption of food material .

 

 

CLS 331:   Lectures Outline

 

Weeks                                               Subjects 

 

1. General introduction

-          Metabolic pathways

-          Catabolism and Anabolism

-          Regulation of metabolic activity 

 

2. Digestion and Absorption

-          Photometry

-          Role of vitamins in metabolism

 

3.Bioenergetics

- The role and chemistry of ATP  

-Standard free energy change of biochemical reactions

 

4.Glycolysis

- Regulatory enzymes of glycolysis

 

5.The citric acid cycle

-Regulation of carbohydrate catabolism

 

6.The Electron Transport chain & oxidative

  phospharylation 

-The energetic value of glucose

 

7.The pentose phosphate shunt and gluconeogenesis

 

8.Glycogen metabolism  

-The glycogen storage diseases

 

9. Oxidative degradation of amino acids

-The synthesis of urea

 

10.Introduction to Inborn Errors of Metabolism

 

11.Oxidation of fatty acids in animal tissue

- B – oxidation pathway

 

12,13.Hormones and their metabolic regulatory role

 

 

CLS 331:   Laboratory Schedule

 

Weeks                                               Subjects

 

1. The hydrolytic activity of salivary amylases on starch 

 

2.Quantitative determination of amylases activity

 

3.The production of pyruvate and acetaldehyde during the

                                  fermentation of glucose by yeast     

 

4. Identification of free amino acids by TLC using silica

                                 gel plates.

 

5.Quantitative determination of protein by the Biuret and

                                 Lowry Reactions

 

6.Enzymatic digestion of fat by pancreatic lipase 

 

7.Effect of Bile salts on pancreatic lipase activity

 

8.Determination of serum urea by the Berthelot reaction

 

9.The determination of the phosphorus content of a nucleic

                                 acid

 

10. Determination of inorganic phosphate  

 

11.Anthrone Reaction 

 

12.Revision

 

13.FINAL LABORATORY EXAMINATION

 

 

 

 

Assessments:

 

First Mid Term Examination:          15       

Second Mid Term Examination:    15

LaboratoryWork and Reports:        15

Final Practical Examination:          15

Final Theoretical Examination:     40

 

 

 

 

 

 

 

References:

 

1. David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, W. H. Freeman; 4th edition (April 23, 2004).

 

1. Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Rodwell, Harper's Biochemistry; McGraw-Hill Publishing Co; 25th edition (August 28, 1999).

 

1. J. G. Salway, Metabolism at a glance; Blackwell Publishers; 2nd edition (August 15, 1999).