CLS 411
Practical Course Specifications
Basic Information
-Title of the programs: CLS
-Title of the course: Clinical Bacteriology 1
-Code number of the course: CLS 411
-Credit hours: 1 hour
-Contact hours: 3 hours
-Academic year: …1432-1433……2st semester
-Level: seven
Overall aim of the course:
1- Learn to identify the given organisms based on their morphology, growth properties and biochemical reaction.
2- Learn how to perform different identification test and understand their principle.
3- Learn about different media used for culturing and their role in identification.
-
Intended learning outcomes of the course: (Skills)
· Practical skills:
1- Perform safe laboratory practice.
2- Learn the aseptic technique and how to apply it to a variety of laboratory techniques.
3- Learn and perform gram stain as many as possible, to be able to master the technique.
4- Learn the proper way to focus a microscope and clean it afterword.
5- Learn and practice the proper streaking of culture plate, and how to obtain isolated colonies.
Interpersonal skills:
1- Learning team work by sharing with other student the reagent and things used in the lab.
2- Learn how to take responsibility on organizing and cleaning their area after they finish work.
- Course contents (Time schedule)
|
Week |
Practical lesson |
No. of hours |
|
1
|
Antibiotic Sensitivity (Sold Method) |
2-3 |
|
2
|
Antibiotic Sensitivity (Broth Dilution Method) |
2-3 |
|
3
|
Staphylococci |
2-3 |
|
4
|
Staphylococci (Continue) |
2-3 |
|
5
|
Streptococci |
2-3 |
|
6
|
Streptococci (Continue) |
2-3 |
|
7 |
Niesseria |
2-3 |
|
8
|
Listeria |
2-3 |
|
9
|
Corynebacterium |
2-3 |
|
10
|
Bacillus |
2-3 |
|
11
|
Mycobaterium |
2-3 |
|
12
|
Revision |
2-3 |
|
13
|
Final Exam |
2 |
|
|
|
|
|
|
|
|
- Teaching Methods:
1- Power point presentation, with pictures of what the student should see in the practical session.
2- Demonstration of gram stain, different culture plates and different test when available.
3- Student should perform gram stain, subculture almost every lab to master the technique.
4- Student should perform different test when possible.
- Assessment methods:
1- Quizzes 10 marks
+ 30 marks for final = total 40 marks for practical.
2- Checking gram stain for student and point out the different mistakes they make.
3- Assessment of the student work in every lab (streaking, focusing of microscope, other test) and coaching.
4- Final spot practical exam.
- References:
1-District Laboratory Practice in Tropical Countries Part 2-2nd Edition.
2-Recommended websites:
· http://pathmicro.med.sc.edu/book/bact-sta.htm.
· www.asm.org. (American Society for Microbiology).