Laboratory Schedule

• Course: CLS 432 Clinical Practice in Biochemistry

Weeks                                               Subjects

 

1,2.Safety procedures :

-       Minimal safety precautions

-       Contaminated equipment / glassware

-       Flammables

-       Chemical hazards

-       Radioisotopes

-       Hepatitis, AIDS etc .

-       Laboratory safety manual

Specimen handling / processing : 

-       Receiving 

-       Master log/ lab. Etc . 

-       Distribution to work areas 

-       Test protocol 

-       Analysis, collection of data 

-       Storage of specimens 

-       Limitations on storage

Quality Control : 

-       Quality control on equipment , reagents , standards etc. 

-       Quality control procedures followed in the laboratory 

-       Review previous 3 months Quality control data (charts,  graphs etc.) 

-       Critique on the procedures followed 

-       Suggestions

Orders, supplies, equipment : 

-       Inventory

-       Orders

-       Supply

Laboratory administration : 

-       Administrative

-       Technical , bench work

-       Space utilization

 

3,4,5.Special chemistry – Radioimmunoassay

 

Lecture and tutorial :

For each test , review of clinical significance, principle employed in a test

Procedure, test protocol , results, interpretation of data , and Quality control  .

Trouble shooting in test procedure / equipment .

 

Orientation to RIA laboratory , test protocols, use of radiolabel material

quality control .

 

RIA of a hormone utilizing the procedure on a protein / steroid hormone

employing a double antibody for separation method. calculation of results

and interpretation of the data .

 

RIA of a hormone employed solid phase method.

Calculation of results and interpretation of the data .

 

6,7,8.Special chemistry – Electrophoresis

 

Lecture tutorial and hospital schedule:

 

Serum protein Electrophoresis :

Procedure , results and interpretation of results .

 

Isoenzymes – lactate dehydrogenate – Enzymology localization , results

and interpretation .

 

Haemoglobin :

Haemoglobinopathies, thalassemias , haemoglobin electrophoresis, results

and interpretation .  

 

 

9-14.Routine chemistry

 

Lecture, tutorial and hospital schedule:

 

Use of automated and other equipment for routine tests performed on

serum , plasma or urine, to include the following :    

 

Glucose, urea, creatinine , sodium , potassium , chloride, CO2 , uric

acid , calcium, CPK, billirubin , albumin , cholesterol , triglycerides,

alkaline phosphates, amylase, acid phosphates, ALT and AST .