CLS 332 Clinical Instrumental Analysis

Course code: 332

Course name : Clinical Instrumental Analysis

Credit hours: 3 theory +1 practical =4

Level: 5

Course Objective:

The primary academic objective to introduce the student to modern methods of instrumental analysis . The focus of the course is in trace analysis, and therefore methods for the identification, separation and quantitation of trace substances will be described. This will include analysis of solids, liquids and vapours. Also to provide a basic understanding of the components, applications, and limitations of modern instruments used in clinical laboratory.

Course Description: The knowledge of the principles of instrumentation is essential for a clinical laboratory technologist which can be utilized in a modern laboratory set up for the collection of clinical data. A good understanding of the principles of instrumentation along with the fundamentals of electronics applicable to laboratory instruments is offered in the course. This will prepare the laboratory technologist to adapt analytical procedures to the instruments of the future.

Course outline:

Laboratory Schedule:

Week	Subjects	Number of week	Number of  hours
1	The visible Absorption Spectrometer	1	1
2	Fluorometric Determination of Riboflavin	1	1
3	Measuring of Osmolality using Digital Micro-Osmometer	1	1
4	Nephelometry and Turbidimetry	1	1
5	Simple Double Beam Spectrometer	1	1
6	Atomic-absorption spectroscopy	1	1
7	Infrared Spectrophotometer	1	1
8	Revision
9	Final Lab Examination

 Methods of evaluation

2 Quizzes	5%
Assignment	3 %
Final exam	12%

 

References:

1. Daniel C. Harris, Quantitative Chemical Analysis, W.H. Freeman & Company; 5th edition (August 1998)

2. Burtis and Ashwood, Saunders, Teitz Foundamentals of Clinical Chemistry, 4th edition, 2000