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Muhammad Farooq khan

Associate Professor

Associate professor of Cell and molecular Biology

كلية العلوم
College of Science, building S5 Lab # 1B44 (zebrafish lab)

Neuro-protective Activity of Pulicaria glutinosa in Oxidative Stress -induced Neurotoxicity in Zebrafish Embryos

, Muhammad Farooq . 2014

Pulicaria glutinosa; Neuro-protectant; Herbal antioxidant

Pulicaria is a genus of flowering plant in the Asteraceae family. It contains over 100 species. In this study the crude extract was prepared from the fresh leaves of P. glutinosa and further fractionated into three solvent to solvent fractions. The zebrafish (Danio rerio) embryos were treated with these extracts to explore the developmental toxicity. The crude extract did not show any significant toxicity; however, hexane fraction induced severe neurotoxicity in zebrafish embryos at 5 μg/mL. The brain formation was severely impeded and sever apoptosis was observed in treated embryos. The methanol fraction on the other hand did not produce any toxicity in zebrafish embryos even at 100 μg/mL. The antioxidant profile revealed that the methanol fraction exhibited highest antioxidant activity followed by chloroform fraction, while hexane fraction did not show any antioxidant activity. Due to high antioxidant activity the methanol fraction was used to rescue the oxidative stress induced neurotoxicity in zebrafish embryos. The methanol fraction not only rescued the neurotoxicity induced by hexane fractions but also protected the ethanol induced neurotoxicity in zebrafish embryos. The neuro-protective effect of methanol fraction could be due to high antioxidant content. This is the first study reporting a new activity of P. glutinosa as neuro-protectant in vivo and a potential therapeutic use in neurodegenerative diseases where pathogenesis is due to oxidative stress. Moreover, this study also reports for the first time the effect of antioxidant activity during embryonic development especially in zebrafish

Publication Work Type
Research article
Volume Number
17
Issue Number
1
Magazine \ Newspaper
International journal of agriculture and biology
Pages
193-98
more of publication
publications

The study was designed to find out the protein complex(s) associated with HDAC3 in liver cancer using a modified form of affinity purification coupled with a mass spectrometry technique in HepG2…

by 15. Muhammad Farooq and Mohammad A.M. Wadaan
2013
publications

Series of Schiff bases of valproic acid hydrazide,

N

-valproylglycine hydrazide and

N

-valproyl-4-ami

-

nobenzoyl hydrazide derivatives were synthesized…

by 13. Ayman El-Faham, Muhammad Farooq, Sherine N. KhattabAhmed M. Elkayal, Mahmoud F. Ibrahim, Nael Abu Taha, Mohammad A. M. Wadaan, Ezaat A. Hamed.
2014
publications

Pulicaria genus belongs to the family Compositae. Some species of this genus have been used in traditional Arab medicine as antispasmodic agent. In this study the Pulicaria glutinosa, which is a…

by 12. Nael Abutaha1, Muhammad Farooq, Fahd Abdu Al-Mekhlafi, Baabbad Almohannad, Sabir Shehata and Mohammad Wadaan
2014