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Dr. rer. nat Elsayed Ahmed Elsayed

Associate Professor

Faculty Staff Member

كلية العلوم
Building 5, AB107
publication
Journal Article
2018

Isolation, Identification and Medium Optimization for Tyrosinase Production by a Newly Isolated Bacillus subtilis NA2 Strain

Danial, Elsayed Ahmed Elsayed, Enas Nabil . 2018

Tyrosinase (EC 1.14.18.1, monophenol, O-diphenol: oxygen oxidoreductase) is a copper-metallo-bifunctional enzyme,
which catalyzes the O-hydroxylation and oxidation of monophenols to quinones. Tyrosinase plays an important role
in the immune defense mechanisms of many prokaryotic and eukaryotic organisms. It also finds many applications in
antioxidants and therapeutic fields. In the present work, a newly isolated tyrosinase-producing strain (Bacillus subtilis
NA2) was identified using 16S rDNA and BLAST analysis. The primary cultivation medium produced tyrosinase at
416.67 U/min/mL with a yield coefficient (YP/X) of 111.75 × 103 U/g cells. Furthermore, the cultivation medium for
the production of tyrosinase was optimized. The final optimized cultivation medium composed of (g/L): sucrose, 10;
KNO3, 10; K2HPO4, 0.5; MgSO4.7H2O, 0.25; tyrosine, 10 and the initial optimized pH of the cultivation medium was
8.5. It has been shown, that the final optimized medium with the optimized pH resulted in an increase of about 39%
in tyrosinase production from the initial un-optimized conditions. The new tyrosinase producing strain was identified
and its volumetric production was significantly increased after the successful optimization of the production medium

Volume Number
8
Issue Number
9
Magazine \ Newspaper
J Appl Phar Sci
Pages
93-101
more of publication
publications

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…

by El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy M
2018
publications

A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
agents. Nitrosation of methyl 5-amino-2-hydroxybenzoate or 1-…

by El-Naggar M, El-Shorbagi A-N, El-Naggar D, Amr AE, Al-Omar M, Elsayed EA
2018
publications

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…

by Elsayed EA, El Enshasy HA
2018