CLS 381 Clinical Analytical Techniques

The course deals with fundamental concepts of quantitative chemical analysis as well as the theoretical principles and operational protocols of instrumental techniques used in the clinical chemistry lab. The introductory part of the course involves molarity and pH calculations, a study of the mechanism of action and preparation of buffers and titrimetric type analysis. Instrumental analytical techniques and components are covered in detail and include Visible and UV spectrophotometry, atomic absorption, fluorescence, flame photometry, mass spectrometry, chemiluminescence, Osmometry and automated analytical systems. Separation methodologies including electrophoresis, different types of chromatography, ion selective electrodes and blood gas analyzers are also covered. In a separate part of the course basic concepts and operational procedures of electron microscopy is studied. Description of the components of both TEM and SEM as well as sample preparation and the development of micrographs are discussed.