Laboratory Skills ( MBIO-240)

1. Introduction to microbiology laboratory skills: laboratory safety, basic equipment, and laboratory techniques

2. Basic chemical preparations: preparation of simple buffers, quantity calculation and PH calibration.

(PPM/Molarity). Preparation and dilution of PPM solutions 

3. Preparation and dilution of Molar solutions 

4. A) Basic aseptic techniques: use of aseptic technique to prevent contamination and maintain sterile conditions in the laboratory. (Sterilization)

B) Microbial growth: culture media (NA/PDA), culture methods (plates/tubes) + different types of media 

5. Pouring media- Microbial isolation from different sources (Bacteria & Fungi).

6. Purification methods (Bacteria & Fungi)- identification (culture characteristic)

7. A) Basic microscopy: principles of light microscopy, types of microscopes, and specimen

B) preparation: +Gram stain and Fungus stain (Lactophenol Cotton Blue). 

8. A) Kirby- Bauer Method (Disc Diffusion Method)

B) Basic bacterial physiology: microbial metabolism, energy production, and microbial metabolic diversity

Microbial enzymatic activity 

- Amylase production test 

- Catalase Activity

9. A) Microbial enumeration: methods for counting viable cell, including plate counts, membrane filtration, and most probable number (MPN) assays.

B) Basic microbiology laboratory calculations: dilutions, serial dilutions, and colony-forming units (CFUs). 

10. Basic molecular techniques: DNA extraction, DNA amplification, agarose gel preparation and gel electrophoresis.