Skip to main content
User Image

Bader Obaid Almutairi

Assistant Professor

Faculty member in Molecular Genetics and epigenetics.

كلية العلوم
College of Sciences No. 5, ground floor, Office No. AB 70
Journal Article

Tantalum doped TiO2 nanoparticles induced cytotoxicity and DNA damage through ROS generation in human neuroblastoma cells

The chemoradiotherapy technique is widely applied in the treatment of cancerous disease. Nowadays, nanoparticles are used in drug delivery and it is essential to confirm the nontoxicity of nano-carriers to improve radiotherapy. So nanoparticles have a great research interest to develop chemo radiotherapies. In this experiment, we aimed to examine the toxic nature of TiO2-Ta nanoparticles on human neuroblastoma (SH-SY5Y) cells. The cytotoxic nature of TiO2-Ta NPs was examined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. We have evaluated the IC50 24 h value of TiO2-Ta nanoparticles as 68 mg/ml by MTT assay. The cytotoxicity of TiO2-Ta nanoparticles was enhanced as the exposure concentration of NPs was increased. Produced oxidative stress such as GSH and LPO were determined and GSH was reduced and LPO was increased as the concentration of TiO2-Ta NPs increased. The Comet test was performed to detect the genotoxic potential of TiO2-Ta nanoparticles. We have noted 34% of DNA damage in SH-SY5Y cells at 300 mg/ml NPs for 24 h. The current study sug- gests that TiO2-Ta NPs induced major ROS-related cytotoxicity and genotoxicity in SH-SY5Y cells. More detailed studies would be needed to confirm the safety issues related to increased application of TiO2-Ta NPs in chemoradiotherapy. Thus this work presents a new type of mesoporous nano-carrier particularly useful for the delivery of safe and effective chemoradiotherapy.

Publication Work Type
Research Article
Publisher Name
King Saud University – Science
more of publication

Cancer is reported to be the leading cause of death and illness worldwide. This research aims to evaluate the phytochemicals, antioxidant, cytotoxic, and apoptotic activities of the polyherbal…

Published in:
Environmental Science and Pollution Research

Cigarette SMOKE (CS) considerably contributes to causing some diseases such as cancer, and it has a role in the alternation of gene expression through several mechanisms including epigenetics…

Published in:

Neuroblastoma is a childhood cancer in which many children still have poor outcomes, emphasising the need to better understand its pathogenesis. Despite recent genome-wide mutation analyses, many…

by Jessica Charlet, Ayumi Tomari, Anthony R. Dallosso, Marianna Szemes, Martina Kaselova, Thomas J. Curry, Bader Almutairi, Heather C. Etchevers, Carmel McConville, Karim T. A. Malik and Keith W. Brown.
Published in: