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Ajamaluddin Malik

Associate Professor

Associate Professor

كلية العلوم
Building no 5, room no 2A56
publication
Journal Article
2023

A common food additive (E452), hexametaphosphate, denatures the digestive enzyme trypsin

Phosphate additions in processed foods are a health risk that has been overlooked. This study examined the
effects of a permitted food additive (E452; Sodium Hexametaphosphate (SHMP)) on trypsin solubility, structure,
and stability at the intestinal physiological pH of 6.0. SHMP-treated trypsin samples were assessed for conformational
changes and aggregation propensity using various spectroscopic and microscopic methods. Far-UV CD
spectroscopy and intrinsic tryptophan fluorescence showed a rise in fluorescence intensity and alpha-helical
structure up to 10 μM SHMP (~1:1 M ratio). Secondary structure loss, tryptophan quenching, and solubility
decrease with an increase in SHMP concentration (>10 μM). SHMP-treated trypsin aggregates showed poor ThT
fluorescence. According to kinetics, SHMP leads to trypsin aggregation instantly. All spectroscopic studies
showed trypsin denatured at above 10 μM SHMP. Thus, digestive enzyme structure and solubility loss will impair
food digestion and health. Therefore, SHMP should be avoided as a food additive.

more of publication
publications

Polyphosphate polymers are chains of phosphate monomers chemically
bonded together via phosphoanhydride bonds. They are found in all prokaryotic and
eukaryotic organisms and are among…

by Ajamaluddin Malik, Javed Masood Khan, Abdulaziz M. Al-Amri, Nojood Altwaijry, Prerna Sharma, Abdullah Alhomida, Priyankar Sen
publications

Phosphate additions in processed foods are a health risk that has been overlooked. This study examined the
effects of a permitted food additive (E452; Sodium Hexametaphosphate (SHMP)) on…

by Ajamaluddin Malik, Javed Masood Khan, Abdulaziz M. Al-Amri, Nojood Altwaijry, Abdullah S Alhomida, Mohammad Shamsul Ola
2023
publications

Protein aggregation is implicated in different human diseases. It also makes the protein less desirable candidate for industry as they exhibit reduced biological activity. Proteins tend to…

by Javed Masood Khan, Ajamaluddin Malik, Abdulaziz M. Al-Amri.
2023
Published in:
Journal of Molecular Liquids