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Dr. Abdulwahed Fahad Alrefaei

Associate Professor

Faculty member in Ecology and Molecular Epidemiology

كلية العلوم
Zoology Department, College of Sciences, ground floor, Office No. AB 58
publication
Journal Article
2021

Microscopic analysis of the inhibition of staphylococcal biofilm formation by Escherichia coli and the disruption of preformed staphylococcal biofilm by bacteriophage

, MANOHARADAS S., ALTAF M., ALREFAEI A., HUSSAIN S., DEVASIA R., BADJAH HADJ A., ABUHASIL M. . 2021

The formation of bacterial biofilms is a severely encountered problem in clinical and industrial settings. Most of the naturally occurring bacterial strains are capable of forming mono or mixed biofilms. In this study, we evaluated the potentiality of three clinically relevant species in forming mono and mixed biofilms over glass surface. In addition, we also appraised the efficiency of bacteriophages in alleviating preformed mono and mixed biofilm. Our initial study focused on the ability of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in forming biofilm on glass cover slip. All the three strains were able to form mono biofilm, although at varying intensities. Interestingly, E. coli inhibited the formation of S. aureus biofilm in a mixed culture. Specific bacteriophages ɸ44AHJD and ɸX174 completely disrupted S. aureus and E. coli preformed biofilm structure after 72 hr of incubation. However, addition of either of the bacteriophage to the mixed E. coli-S. aureus promoted the formation of biofilm by the alternate strain that was not affected by the phage. Our findings elicit the potentiality of common bacterial strains in forming biofilms on smooth glass surface. In addition, these results are very promising for the development of effective drugs using intact bacteriophages for the removal of complicated bacterial biofilms formed in clinically relevant glass surfaces. The observations further complemented the earlier finding of competitive inhibition of S. aureus biofilm development by E. coli.

Volume Number
10
Magazine \ Newspaper
Microscopy Research and Technique
Pages
1-9
more of publication
publications

The current study aimed to examine thymic stromal lymphopoietin receptor (TSLPR) genetic variation and breast cancer (BC) susceptibility in women in Saudi Arabia.

by SEMLALI A., ALMUTAIRI M., ALHARBI S., AL AMRI A., ALREFAEI A. F., ALMUTAIRI B., & ROUABHIA M.
2021
publications

Methylenetetrahydrofolate reductase (MTHFR) polymorphism plays a fundamental role in susceptibility to various diseases, including cancers and autoimmune diseases.

by ALMUTAIRI M., AL-NUMAIR, N., PARINE, N., ALMUTAIRI, B., ALREFAEI, A., ROUABHIA M., SEMLALI, A.
2021
publications

Camptothetin (CPT) is a quinoline alkaloid originally isolated from the Chinese tree, Camptotheca acuminata Decne. CPT was found to have anticancerous and antiviral properties.…

by DEVASIA R., ALTAF M., ALREFAEI A., MANOHARADAS S.
2021