Practical Course ID ZOO 342, Molecular biology

List of Topics  Midterm exam 

Introduction (DNA structures, chromosomes) 

Lab orientation; equipment and tools used 

 DNA extraction from different tissues (the manual method) 

 DNA extraction from different tissues (the kit method) 

 RNA extraction from different tissues 

 Midterm exam 

 Agarose gel electrophoresis 

 Isolation of DNA from the agarose gel 

 SDS-PAGE electrophoresis 

Polymerase Chain Reaction (PCR) 

 Principle of DNA sequencing 

 Final exam