Skip to main content
User Image

Abir Ghannouchi Ben Bacha

Professor

Professor

كلية العلوم
Building 5, 3rd floor, office 285
course

BCH 602

1. Biochemical methods used in next generation sequencing: Basics of NGS chemistry; NGS methods in genomics and transcriptomics which special focus on chip sequencing; Sequencing with low input and simple workflow of DNA and RNA (Ion torrent); throughput capability to sequence multiple strains or disease within fraction of time and cost(Solid platform-); sequencing of wide variety of starting materials, DNA, PCR products, BACS, cDNA and mRNA (454 sequencing).
2. 3D imaging: Biofirming; recent developments; Computer-aided anatomic modelling and 3D reconstruction; Computer-aided tissue classification; Computer-aided tissue implantation; 3D printers
3. Protein crystallization: Properties of proteins and importance of crystallization; Phases of proteins in solution; Nucleation; Supersaturation; Important principles; common methods of crystallization with emphasis on Vapor Diffusion, Slow Evaporation and Dialysis; Ligands and co-crystallization
4. Large scale production and stabilization of recombinant molecules: Recombinant protein expression- advances and challenges; Replicon, promotor, selection marker, affinity tag, tag removal, choosing specific host
5. Macromolecule engineering: Basics of macromolecules and their engineering; Protein engineering; codon bias,inclusion bodies, disulphide bond formation, chaperone expression, protein inactivity.
6. Protein PEGylation: Protein PEGylation Process, Benefits of PEGylation, PEGylation impact on the design of new protein-based medicines
7. Circular dichroism: What is CD? How to study proteins by CD? CD in analysis of protein folding, non folding and misfolding
8. Proteins & Proteomics: What is Proteomics? Determining Protein Structure, Structure and Function Relationships of Proteins, Genomics-Based Predictions of Cellular Proteins, 2D Gel Electrophoresis to Identify Cellular Proteins, Mass Spectrometry to Identify Cellular Proteins, Identifying Protein Interactions, The Yeast Two-Hybrid System, Protein Microarrays, Protein Networks, Proteomes in Different Organisms, Proteomics and Drug Discovery.

course attachements