Morphological and molecular characterization of Aspiculuris tetraptera (nematoda: Heteroxynematidae) from Mus musculus (rodentia: Muridae) in Saudi Arabia.
(2020)., Omer, S. A., Alghamdi, J. M., Alrajeh, A. H., Aldamigh, M., & Mohammed, O. B. . 2020
Aspiculuris tetraptera a pinworm of mice, is an important parasite in institutions with mice
colonies for both research and teaching purposes. Infection with this parasite has impact
on biomedical research. This is likely due to the availability of the parasite’s eggs in the environment,
therefore can easily be transmitted and infection is generally asymptomatic. No
information regarding the prevalence, morphology or phylogeny is available on A. tetraptera
from Saudi Arabia. A group of 50 laboratory mice were investigated for the presence of A.
tetraptera. Worms were described morphologically and molecular characterization was attempted
using 18S rRNA and Cytochrome Oxidase Subunit I genes. The prevalence of A.
tetraptera infestation in the laboratory mice examined was found to be 46%. Morphological
description indicated that the worms belong to A. tetraptera and this was confirmed by
molecular characterization. Both regions studied have shown that the worm under investigation
grouped with A. tetraptera. 18S rDNA sequences obtained in the present study showed
high identity with sequences from A. tetraptera while Cytochrome c Oxidase subunit I gene
(COI) sequences showed intraspecific variation resulted into two haplotypes from the isolates
in the present study. A. tetraptera was recorded for the first time from Saudi Arabia.
Molecular characterization has shown, based on the COI sequences, that the Saudi isolates
of A. tetraptera are distinct.
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