Identification of Nanoscale Ingredients in Commercial Food Products and their Induction of Mitochondrially Mediated Cytotoxic Effects on Human Mesenchymal Stem Cells

مقال فى مجلة
Al-Warthan, Jegan Athinarayanan, Ali A. Alshatwi, Vaiyapuri S. Periasamy andAbdulrahman A. . 2015
نوع عمل المنشور
Journal of Food Science
ملخص المنشورات

Titanium dioxide (E171) and silicon dioxide (E551) are common additives found in food products, personal-care products, and many other consumer products used in daily life. Recent studies have reported that these food additives (manufactured E171 and E551) contain nanosized particles of less than 100 nm. However, the particle size distribution and morphology of added TiO2 and SiO2 particles are not typically stated on the package label. Furthermore, there is an increasing debate regarding health and safety concerns related to the use of synthetic food additives containing nanosized ingredients in consumer products. In this study, we identified the size and morphology of TiO2and SiO2 particles in commercially available food products by using transmission electron microscope (TEM). In addition, the in vitrotoxicological effects of E171 and E551 on human mesenchymal stem cells (hMSCs), an adult stem cell-based model, were assessed using the MTT assay and a flow cytometry-based JC-1 assay. Our TEM results confirmed the presence of nanoscale ingredients in food products, and the in vitro toxicology results indicated that the nanoscale E171 and E551 ingredients induced dose-dependent cytotoxicity, changes in cellular morphology, and the loss of mitochondrial trans-membrane potential in hMSCs. These preliminary results clearly demonstrated that the nanoscale E171 and E551 particles had adverse effects on hMSCs by inducing oxidative stress-mediated cell death. Accordingly, further studies are needed to identify the specific pathway involved, with an emphasis on differential gene expression in hMSCs.