Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae

مقال فى مجلة
نوع عمل المنشور
article
مدينة النشر
Riyadh
اسم الناشر
www.elsevier.com
مجلة/صحيفة
Journal of King Saud University - Science
رقم المجلد
34
الصفحات
101774
ملخص المنشورات

Mannan oligosaccharide is one of the major components of the yeast cell wall. In the present study, the produc- tion, characterization, and bioactivity of yeast mannan were investigated. Four indigenous yeast isolates were obtained from various kinds of juice collected from local markets in Al-Ahsa, KSA, and analyzed using morpho- logical and biochemical methods. Isolate RY1 showed the highest production of mannan. RY1 was identified as Saccharomyces cerevisiae based on morphological characteristics and sequencing of the 18S rRNA gene (GenBank accession number LC479088.1). Mannan-RY1 was characterized by polymer analytical methods, 13C and 1H nu- clear magnetic resonance spectroscopy (NMR), and infrared spectroscopy (IR). Interestingly, the mannan ex- tracted from RY1 showed a significant ability to scavenge hydroxyl radicals and superoxide anions. In addition, mannan was found to have pronounced anti-tumor activity against liver (HepG2) and breast cancer (MCF7) cell lines. The results presented in this study confirm the bio-therapeutic activity of S. cerevisiae mannan, which could be used as a potential drug for cancer treatment