Sub-lethal effect of synthetic pyrethroid pesticide on metabolic enzymes and protein profile of non-target Zebra fish, Danio rerio
Extensive application of pesticides in the agricultural field affects the enzymatic activity of non-target animals,
including fishes. In this study, the impact of sublethal concentration of fenvalerate on marker
enzymes of freshwater Zebrafish was evaluated. Pesticide-induced stress can specifically affect nontarget
fishes, through an elevated level of reactive oxygen species which is responsible for biochemical, cell
metabolism, and physiological activities. The oxidative stress-mediated by fenvalerate at sub-lethal concentrations
after 28 days of exposure to Zebrafish. Following 28 days of exposure to the pesticide, catalase,
superoxide dismutase, aspartate aminotransferases, alanine aminotransferase, alkaline phosphatase, and
acid phosphatase were assessed. Results revealed a reduction of superoxide dismutase activity after
28 days of exposure in a sub-lethal concentration of fenvalerate in the liver and gills. In the liver, catalase activity
was found to be less in fenvalerate exposed fish than in control fish. In the liver, increase of 75.75% aspartate
aminotransferase and a 38% increase in alanine aminotransferase in the gills. SGPT activity was relatively
higher than SGOT suggests more contribution of phyruvalate than oxaloacetate formation. Fenvalerate
induced changes in acid phosphatase and alkaline phosphatase activity in the liver and gills of Zebrafish
after four weeks of exposure. Fenvalerate induced expression of various stress proteins in gill, liver, followed
by muscle. Some proteins lost their intensity due to fenvalerate toxicity. Results revealed that enzyme
assays and SDS-PAGE analysis for protein subunits determination are relevant tools to monitor stress in
freshwater ecosystems. The findings suggest that in monitoring the fenvalerate toxicity program, enzyme
activities can be a potent diagnostic tool for fenvalerate-induced toxicity.
Extensive application of pesticides in the agricultural field affects the enzymatic activity of non-target animals,
including fishes. In this study, the impact of sublethal concentration of…
Fish kidneys are sensitive to chemical changes in the freshwater ecosystem because they are directly and constantly exposed to chemicals dissolved in the water. This study evaluated nephrotoxicity…