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محمد سعود العنزي

Professor

المشرف على كرسي ابحاث الجينوم

كلية العلوم
2ِِA68
المنشورات
مقال فى مجلة
2022
تم النشر فى:

The Expression Patterns of Human Cancer-Testis Genes are Induced Through Epigenetic Drugs in Colon Cancer Cells.

Background: The expression of human germline genes is restricted to the germ cells of the gonads, which produce sperm and eggs. The germline genes involved in testis development and potentially activated in cancer cells are known as cancer-testis (CT) genes. These genes are potential therapeutic targets and biomarkers, as well as drivers of the oncogenic process. CT genes can be reactivated by treatment with drugs that demethylate DNA. The majority of the existing literature on CT gene activation focuses on X-chromosome-produced CT genes. We tested the hypothesis that epigenetic landscape changes, such as DNA methylation, can alter several CT gene expression profiles in cancer and germ cells.

Methods: Colon cancer (CC) cell lines were treated with the DNA methyltransferase inhibitor (DNMTi) 5-aza-2'-deoxycytidine, or with the histone deacetylase inhibitor (HDACi) trichostatin A (TSA). The effects of these epigenetic treatments on the transcriptional activation of previously published CT genes (CTAG1A, SCP2D1, TKTL2, LYZL6, TEX33, and ACTRT1) and testis-specific genes (NUTM1, ASB17, ZSWIM2, ADAM2, and C10orf82) were investigated.

Results: We found that treatment of CC cell lines with 5-aza-2'-deoxycytidine or TSA correlated with activation of X-encoded CT genes and non-X-encoded CT genes in somatic (non-germline) cells.

Conclusion: These findings confirm that a subset of CT genes can be regulated by hypomethylating drugs and subsequently provide a potential therapeutic target for cancer.

Keywords: DNA methyl-transferase inhibitor (DNMTi); cancer-testis (CT) gene; colon cancer; histone deacetylase inhibitor (HDACi); immunotherapy.

نوع عمل المنشور
Research
اسم الناشر
Pharmaceuticals
رقم المجلد
15
الصفحات
14
مزيد من المنشورات
publications

In Saudi Arabia, colon cancer (CC) is the most prevalent cancer in men and the third most common cancer in women. Rather than being detected through screening programs, most CC cases are diagnosed…

بواسطة Mikhlid H. Almutairi, Turki M. Alrubie , Abdullah M. Alamri , Bader O. Almutairi , Abdulwahed F. Alrefaei , Maha M. Arafah , Mohammad Alanazi and Abdelhabib Semlali
2022
تم النشر فى:
Genes
publications

In this study we investigated the effects of multigenerational exposures to acrylamide (ACR) on ovarian function. Fifty-day-old Wistar albino female rats were divided into the control and ACR-…

بواسطة Nouf Aldawood, Maroua Jalouli, Abdulkarem Alrezaki, Saber Nahdi, Abdullah Alamri, Mohamed Alanazi, Salim Manoharadas, Saleh Alwasel, and Abdel Halim Harrath.
2022
تم النشر فى:
Aging
publications

Background: The expression of human germline genes is restricted to the germ cells of the gonads, which produce sperm and eggs. The germline genes involved in testis development…

بواسطة Mikhlid H. Almutairi *, Turki M. Alrubie, Bader O. Almutairi, Abdullah M. Alamri, Abdulwahed F. Alrefaei, Maha M. Arafah, Mohammad Alanazi, Abdelhabib Semlali.
2022
تم النشر فى:
Pharmaceuticals