BCH 590 – Selected Topics in Biochemistry

Selected advanced topics, with emphasis on the latest developments.

The course provides coverage of the major topics in biotechnology, molecular biology and special topics of current interest in Biochemistry

 

• Course Description

Selected advanced topics, with emphasis on the latest developments.

The course provides coverage of the major topics in biotechnology, molecular biology and special topics of current interest in Biochemistry.

• Objectives

This course is designed with the objectives to help the students:

A. Learn the basics of Genetic Engineering

• Understand Recombinant DNA technology
• Learn about DNA cloning and transformation
• Learn about knock down or knock out
• Understand the genome editing technology: CRISPR/Cas9 Genome Editing System and its application
• Understand Genome Alteration technologies such as zinc finger nucleases, TALENs and homologues recombination and the differences between them.
• Appreciate the importance of The Human Genome Project and its contribution to the field of genetic engineering
• Understand site directed mutagenesis and its applications

B. Learn the basics of detecting protein-protein interactions

• Understand protein tagging with fluorophores
• Understand colocalization of proteins
• Understand affinity protein purification
• Understand co-immunoprecipitation and its applications

 

• Reference:

• Relevant Research papers and study material provided by the teacher
• Any other relevant textbook recommended by lecturer