CRISPR-Cas12-Based Rapid Authentication of Halal Food
Deng, Yinhuan Wu, Yi Dong, Yachen Shi, Hao Yang, Jiaqi Zhang, Mohammad Rizwan Khan, Sha Deng, Guiping He, Qiang He, Yuanping Lv, and Ruijie . 2021
The halal food market is globally growing along with the increased risk of adulteration. We proposed an amplificationfree
and mix-to-read CRISPR-Cas12-based nucleic acid analytical strategy allowing rapid identification and analysis of pork
components, thus enriching the toolbox for ensuring halal food authenticity. We designed and optimized guide RNA (gRNA)
targeting the pork cytochrome b (Cyt b) gene. gRNA allowed specific identification of the target Cyt b gene from pork components
followed by activation of Cas12 protein to abundantly cleave single-stranded DNA probes with terminally labeled fluorophore and
quencher groups, thus turning on fluorescence. The presence of the pork Cyt b gene thus can be mix-and-read- and only-one-stepdetected,
which may indicate the risk of halal food adulteration. The method allowed specific discrimination of pork meat from beef,
mutton, and chicken and yielded a detection limit of 2.7 ng/μL of total DNA from pork meat. The reliability of the method was
tested using the following processed meat products: halal foods beef luncheon meat and spiced beef and non-halal foods sausage and dried pork slices. The CRISPR-Cas12-based nucleic acid test strategy is promising for rapid food authentication.
Lead (Pb2+) pollution is a serious food safety issue, rapid detection of Pb2+ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing…
The halal food market is globally growing along with the increased risk of adulteration. We proposed an amplificationfree
and mix-to-read CRISPR-Cas12-based nucleic acid analytical strategy…