Silver nitrate mediated improvement in micropropagation and amelioration of micro‐morpho‐anatomical structures in Oxystelma esculentum (L.f.) Sm.

مقال فى مجلة

2024

تم النشر فى: https://link.springer.com/article/10.1007/s11627-024-10417-0#citeas

The present study highlights the effect of silver nitrate (AgNO3) in the promotion of in vitroregeneration and morpho-structural growth and developments in Oxystelma esculentum(L.f.) Sm. The incorporation of AgNO3 into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentrations of AgNO3 screened, 0.5 mg L−1 AgNO3 along with 0.5 mg L−1 meta-Topolin (mT) and 0.25 mg L−1 indole-3-acetic acid (IAA) stimulated the highest count, and length of shoots (143 shoots and 14.0 cm length) after 3 mo of incubation and the 3rd subculture. The in vitro–induced deformities in the tissues of the control stem and foliages were improved when the shoots were grown in AgNO3-enriched medium. Silver nitrate increased the photosynthetic pigments and improved the formation of functional stomata. The AgNO3-supplemented medium-derived shoots exhibited accelerated adventitious root induction (100% rhizogenesis, 21.0 roots per shoot) when the cut ends of shoots were treated with 200.0 mg L−1 indole-3-butyric acid (IBA) for 5 min under greenhouse conditions. The incorporation of AgNO3 with optimal plant growth regulators in the medium promoted overall growth. It prevented in vitro–induced morphological and anatomical anomalies in the leaf and stem, which considerably improved the greenhouse and in vivo survival of micropropagated plantlets.

• اسم الناشر: Springer Nature
• مجلة/صحيفة: In Vitro Cellular & Developmental Biology - Plant
• الصفحات: https://doi.org/10.1007/s11627-024-10417-0